Characterization of bacteriocin and chitinase producing bacterial isolates with broad-spectrum antimicrobial activities
| Autor: | Muhammad Yasir, Basit Zeshan, Nur Hardy Abu Daud, Izzah Shahid, Hafza Khalid |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Technology General Chemical Engineering Science General Physics and Astronomy Bacillus Chitinase activity medicine.disease_cause Antifungal Microbiology 03 medical and health sciences Broad spectrum 0302 clinical medicine Bacteriocin Bacteriocins medicine General Materials Science General Environmental Science chemistry.chemical_classification Strain (chemistry) biology Chemistry General Engineering Chitinase Pathogenic bacteria Antimicrobial Antibacterial 030104 developmental biology Enzyme Aspergillus 030220 oncology & carcinogenesis biology.protein General Earth and Planetary Sciences |
| Zdroj: | SN Applied Sciences, Vol 3, Iss 8, Pp 1-12 (2021) |
| ISSN: | 2523-3971 2523-3963 |
| Popis: | There is a need for more efficient and eco-friendly approaches to overcome increasing microbial infections. Bacteriocins and chitinases fromBacillusspp. can be powerful alternatives to conventional antibiotics and antifungal drugs, respectively. The purpose of this study was to assess the inhibitory potential of bacteriocins and chitinase enzymes against multiple resistant bacterial and fungal pathogens. Bacterial isolates were selected by growth on minimal salts medium and after that were morphologically and biochemically characterized. The physiochemical characterization of bacteriocins was carried out. The inhibitory potential of bacteriocins towards six pathogenic bacteria was determined by the well diffusion assay while chitinase activity towards three fungal strains was determined by the dual plate culture assay. Two bacterial strains (WW2P1and WRE4P2), out of nine showed inhibition ofK. pneumonia, P. aeruginosa, E. coliand MRSA while WW4P2was positive againstS. typhimuriumandE. coliand WRE10P2againstP. aeruginosa,S. pneumoniae. Two bacterial isolates (WW3P1and WRE10P2) were chosen for further study on the basis of their antifungal activities. Of these, WW3P1 isolate was more effective against A. fumigatus as well as A. niger. The proteinaceous nature of the bacteriocins was confirmed by treatment of the crude extract with proteinase K. It was found that the inhibitory activity of strain WW3P1 against E. coli was highest at 20 °C, and againstS. pneumoniae it was at 20 °C and pH 10 after treatment with EDTA. Inhibition by strain the WRE10P2against P. aeruginosa was highest at 20 °C and pH 14. It was found that EDTA increased the inhibitory activity of strain WW2P1againstP. aeruginosa,K. pneumoniaeandE. coliby 2 ± 0.235, 3.5 ± 0.288, 2.5 ± 1.040 times, respectively, of strain WRE4P2againstP. aeruginosaandE. coliby 2.5 ± 0.763, 2.7 ± 0.5 times, respectively, and of strain WRE10P2againstS. pneumoniaeby 3 ± 0.6236 times. The isolates have promising inhibitory activity, which should be further analyzed for the commercial production of antimicrobials.Article highlightsThe current study aimed to isolate the microbiome from wheat plant (Triticum aestivumL.), to screen for bacteriocin production and to assess its antimicrobial activity against human pathogens.Forty-one phenotypically different bacterial colonies were subjected to bacteriocin purification from which 25 colonies showed positive reactions.These 25 bacterial isolates were screened against six different human bacterial pathogens using the well diffusion method to check the antimicrobial activity. Out of nine bacterial isolates, WW3P1 and WRE10P2 were able to degrade the chitin and utilize it as their sole energy source. Strain WRE4P2 exhibited partial inactivation in its activity against MRSA after treatment with proteinase K. |
| Databáze: | OpenAIRE |
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