Polycystic Ovary Syndrome and Insulin Physiology: An Observational Quantitative Serum Proteomics Study in adolescent, Normal-Weight Females
| Autor: | Diana J. Garay-Baquero, Flora Bacopoulou, Shaun Sabico, Nikos Athanasopoulos, Nasser M. Al-Daghri, Majed S. Alokail, Spiros D. Garbis, George P. Chrousos, Antigoni Manousopoulou, Efthymios Deligeoroglou, Amal Alenad, Jie Teng |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proteomics Adolescent medicine.medical_treatment Clinical Biochemistry Quantitative proteomics Physiology Overweight Serology 03 medical and health sciences Young Adult Insulin resistance Medicine Endocrine system Humans Insulin 030102 biochemistry & molecular biology business.industry Body Weight medicine.disease Polycystic ovary Obesity 030104 developmental biology Female medicine.symptom business Polycystic Ovary Syndrome |
| Popis: | Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with insulin resistance, even in the absence of overweight/obesity. The aim of the present study is to examine the global serum proteomic profile of adolescent, normal-weight females with PCOS in order to gain novel insight in the association of this endocrine disorder with insulin physiology and to identify novel circulating markers that can guide intervention protocols. Methods: Non-depleted serum from normal-weight (BMI: 18–23 kg m −2 ), adolescent females (13–21 years old) with PCOS (n = 20) is compared to BMI- and age-matched healthy controls (n = 20) using our 3D quantitative proteomics methodology. Serum samples from study participants are randomly pooled to form four biological replicates of females with PCOS and four of healthy controls (n = 5 per sample pool). Results: One-hundred and twenty-six proteins are differentially expressed in females with PCOS compared to controls. Gene ontology analysis shows significant enrichment for terms related to inflammatory immune response, metabolism and insulin-like growth factor receptor signaling pathway. Circulating levels of IGF-1 and -2 and IGFBP-2, -3, and -4 are found to be lower in females with PCOS compared to healthy controls. Conclusions: The present serum proteomics study provides insight into the pro-inflammatory status and insulin dysregulation in young females with PCOS and identifies potential serological markers that can guide early intervention protocols. |
| Databáze: | OpenAIRE |
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