Dietary magnesium supplementation prevents and reverses vascular and soft tissue calcifications in uremic rats
| Autor: | Mariano Rodriguez, Paula Buendía, Escolastico Aguilera-Tejero, Carmen Herencia, Antonio Canalejo, Alan Peralta-Ramírez, Carmen Pineda, Andrés Carmona, Juan F. Alcala-Diaz, João M. Frazão, M. Victoria Pendón-Ruiz de Mier, Addy Montes de Oca, Julio M. Martinez-Moreno, Juan R. Muñoz-Castañeda, Noemi Vergara, Ignacio González López, Juan M. Díaz-Tocados, Sonja Steppan, Julia Carracedo, Yolanda Almaden, Ana I. Raya, María E. Rodríguez-Ortiz, Arnold J. Felsenfeld |
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| Rok vydání: | 2017 |
| Předmět: |
Male
medicine.medical_specialty medicine.drug_class 030232 urology & nephrology Renal function chemistry.chemical_element 030204 cardiovascular system & hematology Nephrectomy Bone and Bones Umbilical vein Phosphates 03 medical and health sciences Basal (phylogenetics) 0302 clinical medicine Internal medicine Human Umbilical Vein Endothelial Cells Animals Humans Medicine Magnesium Rats Wistar Vascular Calcification Chelating Agents Uremia business.industry Soft tissue Rats Phosphate binder Disease Models Animal Blood pressure Endocrinology chemistry Nephrology Dietary Supplements business Homeostasis |
| Zdroj: | Kidney International. 92:1084-1099 |
| ISSN: | 0085-2538 |
| DOI: | 10.1016/j.kint.2017.04.011 |
| Popis: | Although magnesium has been shown to prevent vascular calcification in vitro , controlled in vivo studies in uremic animal models are limited. To determine whether dietary magnesium supplementation protects against the development of vascular calcification, 5/6 nephrectomized Wistar rats were fed diets with different magnesium content increasing from 0.1 to 1.1%. In one study we analyzed bone specimens from rats fed 0.1%, 0.3%, and 0.6% magnesium diets, and in another study we evaluated the effect of intraperitoneal magnesium on vascular calcification in 5/6 nephrectomized rats. The effects of magnesium on established vascular calcification were also evaluated in uremic rats fed on diets with either normal (0.1%) or moderately increased magnesium (0.6%) content. The increase in dietary magnesium resulted in a marked reduction in vascular calcification, together with improved mineral metabolism and renal function. Moderately elevated dietary magnesium (0.3%), but not high dietary magnesium (0.6%), improved bone homeostasis as compared to basal dietary magnesium (0.1%). Results of our study also suggested that the protective effect of magnesium on vascular calcification was not limited to its action as an intestinal phosphate binder since magnesium administered intraperitoneally also decreased vascular calcification. Oral magnesium supplementation also reduced blood pressure in uremic rats, and in vitro medium magnesium decreased BMP-2 and p65–NF-κB in TNF-α–treated human umbilical vein endothelial cells. Finally, in uremic rats with established vascular calcification, increasing dietary magnesium from 0.1% magnesium to 0.6% reduced the mortality rate from 52% to 28%, which was associated with reduced vascular calcification. Thus, increasing dietary magnesium reduced both vascular calcification and mortality in uremic rats. |
| Databáze: | OpenAIRE |
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