Induction of Beige-Like Adipocytes in 3T3-L1 Cells
| Autor: | Ken Kato, Hiroki Asano, Masayuki Funaba, Takayuki Y. Nara, Tohru Matsui, Yohei Kanamori, Satoshi Higurashi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Stromal cell IBMX Ucp1 Cellular differentiation Adipocytes White White adipose tissue Biology adipocyte Real-Time Polymerase Chain Reaction Biochemistry Ion Channels Mitochondrial Proteins Rosiglitazone chemistry.chemical_compound Mice Internal medicine Adipocyte 1-Methyl-3-isobutylxanthine 3T3-L1 Cells medicine Animals Uncoupling Protein 1 cell culture General Veterinary Full Paper Cell Differentiation beige adipocyte Thermogenin cellular differentiation Endocrinology chemistry Adipogenesis Cell culture RNA Triiodothyronine Thiazolidinediones Transcription Factors |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 0916-7250 |
| Popis: | There are two types of brown adipocytes: classical brown adipocytes that form the brown fat depots and beige adipocytes that emerge in the white fat depots. Beige adipocytes have a low level of uncoupling protein 1 (Ucp1) expression in the basal state, but Ucp1 expression is increased in response to β adrenergic receptor activation. The present study explored the factors responsible for the differentiation of 3T3-L1 white preadipocytes to beige adipocytes. Significant expression of Ucp1 was not detected under any tested conditions in the absence of isoproterenol (Iso), an agonist of β adrenergic receptor. Iso-induced Ucp1 expression was significantly higher in the cells treated with a mixture of triiodothyronine (T3) and 3-isobutyl-1-methylxanthine (IBMX) for days 0–8 than in the control cells. Chronic IBMX treatment was indispensable for the enhanced Iso-induced Ucp1 expression, and treatment with additional rosiglitazone (Rosi) for days 0–8 further increased the Ucp1 expression. Recently, genes were identified that are predominantly expressed in beige adipocytes, which were induced from stromal vascular cells in white fat depots. However, the expression levels of the beige adipocyte-selective genes in the adipocytes induced by the mixture of T3, IBMX and Rosi did not differ from those in the control adipocytes. The present study indicates that 3T3-L1 cells can differentiate to beige-like adipocytes by prolonged treatment with the mixture of T3, IBMX and Rosi and that the gene expression profile of the adipocytes is distinct from those previously induced from white fat depots. |
| Databáze: | OpenAIRE |
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