MiR-145 functions as a tumor suppressor in Papillary Thyroid Cancer by inhibiting RAB5C
| Autor: | Xudong Zhao, Tianshu Li, Wenyue Ji, Ting Liu, Wei Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male endocrine system diseases Cell Down-Regulation Apoptosis law.invention Metastasis Papillary thyroid cancer Thyroid carcinoma 03 medical and health sciences 0302 clinical medicine law Cell Movement Cell Line Tumor microRNA medicine Humans Genes Tumor Suppressor Thyroid Neoplasms Cell Proliferation rab5 GTP-Binding Proteins business.industry Thyroid RAB5C General Medicine Middle Aged medicine.disease miR-145 Gene Expression Regulation Neoplastic MicroRNAs medicine.anatomical_structure PTC Thyroid Cancer Papillary Cancer research papillary thyroid carcinoma Suppressor 030211 gastroenterology & hepatology Female business Research Paper |
| Zdroj: | International Journal of Medical Sciences |
| ISSN: | 1449-1907 |
| Popis: | Papillary thyroid carcinoma (PTC) accounts for the largest proportion of thyroid cancers; and its morbidity rate has dramatically increased in recent decades. However, the pathogenesis mechanisms of PTC are still not clear. This study aimed to reveal that miR-145 acts as an antitumor miRNA in the progression of PTC. In the present study, the expression of miR-145 was analyzed in 57 paired PTC patient samples. The relationship between clinicopathological features and miR-145 expression were also defined. The tumor suppressive function of miR-145 on PTC cell metastasis, proliferation and apoptosis were revealed in vitro. Also, we used dual luciferase reporter assay to define the relationship of miR-145 and RAB5C. RAB5C was reported to participate in cell invasion and cell motility. We found that miR-145 was downregulated in PTCs, which was negatively correlated with PTC progression and metastasis. MiR-145 inhibited PTC migration, proliferation and promoted apoptosis by directly suppresing RAB5C. In conclusion, miR-145 functions as a tumor suppressor in PTC by inhibiting RAB5C. MiR-145 and RAB5C are potential therapeutic targets in therapy of aggressive PTC cases. |
| Databáze: | OpenAIRE |
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