Production and characterization of monoclonal antibodies specific for Mycobacterium bovis
| Autor: | L.E. Cottis, Alan Radford, J.L. Ripper, P. R. Wood, P. Plackett, M. John, P.G. Bundesen, D. B. Rylatt |
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| Rok vydání: | 1988 |
| Předmět: |
Tuberculosis
Anticorps monoclonal medicine.drug_class Blotting Western Enzyme-Linked Immunosorbent Assay Cross Reactions Monoclonal antibody Microbiology Mycobacterium Epitopes Mice Antibody Specificity medicine Animals Humans Mycobacterium bovis Mice Inbred BALB C biology Antibodies Monoclonal Elisa assay biology.organism_classification medicine.disease Virology Blot Cattle Bacteria |
| Zdroj: | Journal of general microbiology. 134(9) |
| ISSN: | 0022-1287 |
| Popis: | Summary: A series of monoclonal antibodies (MAbs), specific for Mycobacterium bovis and BCG strains, were tested extensively for cross-reactivity to a wide range of mycobacterial species using ELISA, Western blotting and dot-blot analysis. The MAbs bound specifically to M. bovis and BCG and showed limited cross-reactivity with some strains of M. tuberculosis. All these MAbs recognized a 22 kDa protein previously termed MPB70, and by competitive ELISA analysis appeared to detect at least three M. bovis-specific determinants on the MPB70 molecule. |
| Databáze: | OpenAIRE |
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