Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells
Autor: | Feng-Chiao Tsai, Mingyu Chung, Arnold Hayer, Lin Shao, Anjali Bisaria, Eric Betzig, Hee Won Yang, Tobias Meyer, Lydia-Marie Joubert |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Mitosis Cell Count Walking Cell junction Models Biological Actin-Related Protein 2-3 Complex Article Polymerization Cell membrane 03 medical and health sciences Imaging Three-Dimensional Phagocytosis Antigens CD Cell Movement Cell polarity medicine Human Umbilical Vein Endothelial Cells Humans Pseudopodia Actin Cadherin Chemistry Cell Membrane Cell Polarity Endothelial Cells Cell migration Catenins Cell Biology Actomyosin Cadherins Cell biology Optogenetics 030104 developmental biology medicine.anatomical_structure HEK293 Cells Intercellular Junctions Catenin |
Popis: | The development and maintenance of tissues requires collective cell movement, during which neighbouring cells coordinate the polarity of their migration machineries. Here, we ask how polarity signals are transmitted from one cell to another across symmetrical cadherin junctions, during collective migration. We demonstrate that collectively migrating endothelial cells have polarized VE-cadherin-rich membrane protrusions, 'cadherin fingers', which leading cells extend from their rear and follower cells engulf at their front, thereby generating opposite membrane curvatures and asymmetric recruitment of curvature-sensing proteins. In follower cells, engulfment of cadherin fingers occurs along with the formation of a lamellipodia-like zone with low actomyosin contractility, and requires VE-cadherin/catenin complexes and Arp2/3-driven actin polymerization. Lateral accumulation of cadherin fingers in follower cells precedes turning, and increased actomyosin contractility can initiate cadherin finger extension as well as engulfment by a neighbouring cell, to promote follower behaviour. We propose that cadherin fingers serve as guidance cues that direct collective cell migration. |
Databáze: | OpenAIRE |
Externí odkaz: |