A pilot study of cytokine profile in cerebrospinal fluid of children with acute lymphocytic leukemia and neurotoxic side effects of chemotherapy
| Autor: | O V Koryakina, A Yu Maksimova, Larisa Polushina, Olga P. Kovtun, V V Bazarnyi |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Chemokine
Chemotherapy biology business.industry medicine.medical_treatment Neurotoxicity Pilot Projects Stem cell factor General Medicine Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease General Biochemistry Genetics and Molecular Biology Peripheral neuropathy Cerebrospinal fluid Acute lymphocytic leukemia Acute Disease Immunology medicine biology.protein Cytokines Humans Prospective Studies Child business Prospective cohort study |
| Zdroj: | Biomeditsinskaya Khimiya. 67:374-377 |
| ISSN: | 2310-6905 2310-6972 |
| Popis: | In some cases standard chemotherapy of acute lymphocytic leukemia (ALL) leads to neurotoxicity; its mechanisms, methods of prognosis, and prevention are being actively studied. The aim of this study was to assess the cytokine profile in cerebrospinal fluid (CSF) of children with ALL and neurotoxic side effects of chemotherapy. This prospective study included 24 children with ALL aged from 3 to 17 years. Patients were further subdivided into ALL patients with (main group) and without neurological complications (comparison group). The level of cytokines in CSF was measured by Xmap technology (Luminex) using Invitrogen test systems (eBioscience) and the Luminex 200 system. The comparative analysis of the cytokine profile in the group of children with chemotherapy-induced neurotoxic complications revealed elevated levels of chemokine CXCL12 (SDF-1α) and stem cell factor (SCF). Increased level of these cytokines in CSF was characterized by a relatively risk for development of toxic peripheral neuropathy.Standartnaia khimioterapiia ostrogo limfoblastnogo leĭkoza (OLL) privodit v riade sluchaev k neĭrotoksicheskim oslozhneniiam, mekhanizmy kotorykh, sposoby prognozirovaniia i profilaktiki aktivno izuchaiutsia. Tsel' dannoĭ raboty — otsenit' tsitokinovyĭ spektr likvora pri neĭrotoksicheskikh oslozhneniiakh khimioterapii u deteĭ s OLL. V prospektivnoe issledovanie vkliucheno 24 rebenka s diagnozom OLL v vozraste ot 3 do 17 let, kotorye byli podrazdeleny na dve gruppy — s nevrologicheskimi oslozhneniiami (osnovnaia gruppa) i bez nevrologicheskikh oslozhneniĭ (gruppa sravneniia). Uroven' tsitokinov v likvore opredeliali metodom mul'tiparametricheskogo fluorestsentnogo analiza s primeneniem magnitnykh mikrosfer (Xmap-tekhnologiia, Luminex) s ispol'zovaniem test-sistem Invitrogen (eBioscience) i mul'tipleksnogo analizatora Luminex 200. Pri sravnitel'nom analize tsitokinovogo profilia v gruppe deteĭ s khimio-indutsirovannymi neĭrotoksicheskimi oslozhneniiami bylo vyiavleno povyshenie urovnia khemokina CXCL12 (SDF-1α) i faktora stvolovykh kletok (SCF). Uvelichenie kontsentratsii étikh tsitokinov v likvore svidetel'stvovalo ob otnositel'no vysokom riske razvitiia toksicheskoĭ perifericheskoĭ polinevropatii. |
| Databáze: | OpenAIRE |
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