Arachidonic acid actions on functional integrity and attenuation of the negative effects of palmitic acid in a clonal pancreatic β-cell line

Autor:	Noel G. Morgan, Philip Newsholme, Shalinee Dhayal, Deirdre Keane, Rui Curi, Hilton Kenji Takahashi
Rok vydání:	2010
Předmět:	Palmitates AA arachidonic acid Ppara peroxisome-proliferator-activated receptor α medicine.disease_cause iNOS inducible NO synthase Palmitic acid TNB 2-nitro-5-thiobenzoic acid chemistry.chemical_compound Lipoxygenase Gck glucokinase Insulin-Secreting Cells palmitic acid WST-1 water-soluble tetrazolium salt 1 Insulin DHE dihydroethidine Lipoxygenase Inhibitors Cells Cultured Arachidonic Acid NADPH oxidase biology NEFA non-esterified fatty acid Reverse Transcriptase Polymerase Chain Reaction General Medicine lipotoxicity Peroxisome Glutathione T2DM Type 2 diabetes mellitus NS-398 N-(2-cyclohexyloxyl-4 nitrophenyl)methanesulfonamide Saturated fatty acid Arachidonic acid Ampk AMP-activated protein kinase NF-κB nuclear factor κB UCP uncoupling protein Research Article medicine.medical_specialty Cell Survival LT leukotriene SC-560 5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-(trifluoromethyl)-1H-pyrazole Scd-2 stearoyl-CoA desaturase-2 S5 GSIS glucose-stimulated insulin secretion PI propidium iodide COX cyclo-oxygenase PA palmitic acid RT–PCR (reverse transcription–PCR ROS reactive oxygen species FBS fetal bovine serum HETE hydroxyeicosatetraenoic acid Internal medicine Type 2 diabetes mellitus medicine Animals Humans Cyclooxygenase Inhibitors PG prostaglandin Nitrites pancreatic β-cell Ppard peroxisome-proliferator-activated receptor δ Srebp-1c sterol-regulatory-element-binding protein-1c Fatty acid metabolism PLA2 phospholipase A2 Ech1 peroxisomal Δ3 5 Δ2 4-dienoyl-CoA isomerase WT wild-type Oxidative Stress Endocrinology Diabetes Mellitus Type 2 Gene Expression Regulation chemistry Cyclooxygenase 2 Abcg1 ATP-binding cassette subfamily G member 1 Cyclooxygenase 1 biology.protein Reactive Oxygen Species Oxidative stress
Zdroj:	Clinical Science (London, England : 1979)
ISSN:	1470-8736 0143-5221
DOI:	10.1042/cs20100282
Popis:	Chronic exposure of pancreatic β-cells to saturated non-esterified fatty acids can lead to inhibition of insulin secretion and apoptosis. Several previous studies have demonstrated that saturated fatty acids such as PA (palmitic acid) are detrimental to β-cell function compared with unsaturated fatty acids. In the present study, we describe the effect of the polyunsaturated AA (arachidonic acid) on the function of the clonal pancreatic β-cell line BRIN-BD11 and demonstrate AA-dependent attenuation of PA effects. When added to β-cell incubations at 100 μM, AA can stimulate cell proliferation and chronic (24 h) basal insulin secretion. Microarray analysis and/or real-time PCR indicated significant AA-dependent up-regulation of genes involved in proliferation and fatty acid metabolism [e.g. Angptl (angiopoietin-like protein 4), Ech1 (peroxisomal Δ3,5,Δ2,4-dienoyl-CoA isomerase), Cox-1 (cyclo-oxygenase-1) and Cox-2, P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea47bd0944a23405234a6696ff9cf4eb https://doi.org/10.1042/cs20100282 Zobrazit plný text záznamu