Oxidative stress during post-hypoxic-ischemic reperfusion in the newborn lamb: the effect of nitric oxide synthesis inhibition
| Autor: | Howard M Berger, C.A. Dorrepaal, Ralf M. W. Moison, M. van de Bor, Paul Steendijk, M. Shadid, F van Bel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Antioxidant
medicine.medical_treatment Drug Evaluation Preclinical Diseases Infant Premature Diseases Nitroarginine Infant Newborn Diseases Antioxidants Brain Ischemia Lipid peroxidation chemistry.chemical_compound Central Nervous System Diseases Enzyme Inhibitors Hypoxia Brain GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Bronchopulmonary Dysplasia Netherlands Asphyxia Neonatorum Respiratory Distress Syndrome Brain Malondialdehyde Reperfusion Injury Anesthesia Dehydroascorbic acid Oxidation-Reduction Vitamin medicine.medical_specialty Neurophysiology Disturbances of cerebral development in the young child Nitric oxide Internal medicine medicine Hemorrhagic Disease of Newborn Animals Premature Sheep Dose-Response Relationship Drug business.industry Microcirculation Infant Ascorbic acid Newborn Oxidative Stress Endocrinology Animals Newborn chemistry Pediatrics Perinatology and Child Health Cerebrale ontwikkelingsstoornissen bij het jonge kind Uric acid Lipid Peroxidation Nitric Oxide Synthase business |
| Zdroj: | Pediatric Research, 41, 3, pp. 321-326 Pediatric Research, 41, 321-326 |
| ISSN: | 0031-3998 |
| Popis: | Post-hypoxic-ischemic (HI) reperfusion induces endothelium and neurons to produce excessive amounts of nitric oxide and superoxide, leading to peroxynitrite formation, release of protein-bound metal ions (i.e. iron), and cytotoxic oxidants. We produced severe HI in 18 newborn lambs and serially determined plasma prooxidants (non-protein-bound iron), lipid peroxidation (malondialdehyde), and antioxidative capacity [ratio of ascorbic acid/dehydroascorbic acid (AA/DHA), alpha-tocopherol, sulfhydryl groups, allantoin/uric acid ratio, and vitamin A] in blood effluent from the brain before and at 15, 60, 120, and 180 min after HI. The lambs were divided in three groups: six received a placebo (CONT), six received low dose (10 mg/kg/i.v.) N omega-nitro-L-arginine (NLA-10) to block nitric oxide production, and six received high dose NLA (40 mg/kg/i.v.; NLA-40), immediately after completion of HI. Non-protein-bound iron increased in all groups after HI but was significantly lower in both NLA groups at 180 min post-HI (p < 0.05), the AA/DHA ratio showed a consistent decrease in CONT (at 60 min post-HI, p < 0.05), but remained stable in NLA lambs. alpha-Tocopherol decreased steadily in the CONT, but not in the NLA lambs [180 post-H: 1.9 +/- 0.9 versus 4.2 +/- 0.7 microM (NLA-40), p < 0.05). Malondialdehyde was significantly higher in CONT lambs 120 min post-H compared with NLA groups [0.61 +/- 017 versus 0.44 +/- 0.05 microM (NLA-40), p < 0.05]. Vitamin A and sulfhydryl groups did not differ among groups. We conclude that post-H inhibition of nitric oxide synthesis diminishes non-protein-bound iron increment and preserves antioxidant capacity. |
| Databáze: | OpenAIRE |
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