Differential Expression Patterns of β-Actin mRNA in Cells Undergoing Apoptosis
| Autor: | H. Naora |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Time Factors
Transcription Genetic Actin mrna Biophysics Ionophore Gene Expression Apoptosis Cycloheximide Biology Microfilament Biochemistry Cell Line chemistry.chemical_compound Leukemia Promyelocytic Acute Tumor Cells Cultured Humans Amnion RNA Messenger Differential expression Molecular Biology U937 cell Cell Biology Blotting Northern Molecular biology Actins Cell biology Kinetics chemistry Mrna level Dactinomycin Lymphoma Large B-Cell Diffuse DNA Probes |
| Zdroj: | Biochemical and Biophysical Research Communications. 211:491-496 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1995.1840 |
| Popis: | Microfilaments play an important role in the formation of apoptotic bodies. This study examined expression patterns of beta-actin, a key component of microfilament, during apoptosis. The data suggest that continuous beta-actin expression is not required for formation of apoptotic bodies. beta-actin mRNA levels rapidly declined in HL-60 cells following induction of apoptosis by actinomycin D, but were transiently elevated by two other apoptosis-inducing agents, cycloheximide and ionophore A23187. Furthermore, changes in beta-actin mRNA levels were less marked in U937 cells in which apoptosis was also induced by actinomycin D, as well as in WISH cells which demonstrated no typical apoptotic characteristics following actinomycin D treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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