Staphylococcus aureus internalisation in mast cells in nasal polyps - characterisation of interactions and potential mechanisms
| Autor: | Stephen M. Hayes, Rami J. Salib, Simon P. Goldie, Sylvia L.F. Pender, Raymond N. Allan, Philip G. Harries, Andrew F. Walls, T.C. Biggs |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Staphylococcus aureus Phagocytosis media_common.quotation_subject Immunology Enterotoxin B In situ hybridization medicine.disease_cause Cell Line Microbiology Tissue Culture Techniques Enterotoxins Nasal Polyps medicine Extracellular otorhinolaryngologic diseases Humans Immunology and Allergy Nasal polyps Mast Cells Prospective Studies Internalization Aged media_common Colony-forming unit Chemistry Middle Aged Mast cell medicine.disease Chronic rhinosinusitis medicine.anatomical_structure Mast cells Female |
| Popis: | The file attached to this record is the author's final peer reviewed version. The Publisher's final version can be found by following the DOI link. Background: Chronic rhinosinusitis (CRS) with nasal polyps is a common chronic condition. The exact cause of nasal polyps remains unknown. Recently, we made the novel observation of intracellular localization of Staphylococcus aureus within mast cells in nasal polyps. Objective: This follow-up study aimed to further characterize interactions between S aureus and mast cells in this setting and elucidate potential internalization mechanisms with particular emphasis on the role of staphylococcal enterotoxin B (SEB). Methods: A prospective study was performed using an explant tissue model with ex vivo inferior turbinate mucosa obtained from patients with chronic rhinosinusitis with nasal polyps (n = 7) and patients without CRS (n = 5). Immunohistochemistry was used to characterize S aureus uptake into mast cells and investigate the effects of SEB on this process. An in vitro cell-culture model was used to investigate mast cell–S aureus interactions by using a combination of fluorescent in situ hybridization, confocal laser scanning microscopy, scanning electron microscopy, transmission electron microscopy, and proliferation assays. Results: S aureus was captured by extracellular traps and entered mast cells through phagocytosis. Proliferating intracellular S aureus led to the expansion and eventual rupture of mast cells, resulting in release of viable S aureus into the extracellular space. The presence of SEB appeared to promote internalization of S aureus into mast cells. Conclusion: This study provides new insights into the interactions between S aureus and mast cells, including the internalization process, and demonstrates a prominent role for SEB in promoting uptake of the bacteria into these cells. |
| Databáze: | OpenAIRE |
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