CircHIPK3 sponges miR‐558 to suppress heparanase expression in bladder cancer cells
Autor: | Liang Wang, Dan Tao, Fuqing Zeng, Guosong Jiang, Xingyuan Xiao, Chao Huang, Dong Liu, Yawei Li, Fei Xie, Fuxin Zheng, Miao Wang |
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Jazyk: | angličtina |
Rok vydání: | 2022 |
Předmět: |
0301 basic medicine
Angiogenesis Down-Regulation Biology Protein Serine-Threonine Kinases urologic and male genital diseases Biochemistry Metastasis 03 medical and health sciences 0302 clinical medicine Circular RNA Cell Line Tumor microRNA Gene expression Genetics medicine Humans Heparanase RNA Neoplasm Molecular Biology Cell Proliferation Glucuronidase Bladder cancer Neovascularization Pathologic Sequence Analysis RNA Intracellular Signaling Peptides and Proteins Cancer Articles RNA Circular medicine.disease Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Urinary Bladder Neoplasms 030220 oncology & carcinogenesis Immunology Cancer research RNA Corrigendum |
Zdroj: | EMBO Rep |
Popis: | Increasing evidences suggest that circular RNAs (circRNAs) exert crucial functions in regulating gene expression. In this study, we perform RNA‐seq and identify 6,154 distinct circRNAs from human bladder cancer and normal bladder tissues. We find that hundreds of circRNAs are significantly dysregulated in human bladder cancer tissues. We further show that circHIPK3, also named bladder cancer‐related circular RNA‐2 (BCRC‐2), is significantly down‐regulated in bladder cancer tissues and cell lines, and negatively correlates with bladder cancer grade, invasion as well as lymph node metastasis, respectively. Over‐expression of circHIPK3 effectively inhibits migration, invasion, and angiogenesis of bladder cancer cells in vitro and suppresses bladder cancer growth and metastasis in vivo . Mechanistic studies reveal that circHIPK3 contains two critical binding sites for the microRNA miR‐558 and can abundantly sponge miR‐558 to suppress the expression of heparanase (HPSE). Taken together, our findings provide evidence that circRNAs act as “microRNA sponges”, and suggest a new therapeutic target for the treatment of bladder cancer. |
Databáze: | OpenAIRE |
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