Central giant cell granulomas of the jaws stromal cells harbour mutations and have osteogenic differentiation capacity, in vivo and in vitro

Autor: Lucyene Miguita, Juliana Cristina de Souza, Victor Coutinho Bastos, Nubia Braga Pereira, Raphaela Alvarenga Braga de Freitas, Leticia Martins Guimarães, Gleide Fernandes de Avelar, Luciana Oliveira Andrade, Walderez Ornelas Dutra, Fábio Daumas Nunes, Wagner Henriques Castro, Julio Cesar Tanos de Lacerda, Amanda Maria Sena Reis, Vanessa Fátima Bernardes, Marina Gonçalves Diniz, Ricardo Santiago Gomez, Carolina Cavalieri Gomes
Rok vydání: 2022
Předmět:
Zdroj: Journal of Oral Pathology & Medicine. 51:206-216
ISSN: 1600-0714
0904-2512
DOI: 10.1111/jop.13274
Popis: Central giant cell granulomas (CGCG) of the jaws are osteolytic lesions that may behave aggressively and respond poorly to surgery. Microscopically, in addition to giant cells, there is a mononuclear cell population composed of macrophage/monocytic cells and spindle-shaped cells of mesenchymal origin. Seventy two percent of these tumours harbour mutually exclusive TRPV4, KRAS and FGFR1 mutations. We aimed to assess the mutational status of mononuclear and giant cells and the osteogenic potential of stromal cells in vitro and in vivo.We screened CGCG for signature mutations and used laser-capture microdissection to demonstrate that the mutations are restricted to the mononuclear cells. Additionally, we established CGCG primary cell culture and observed that the cells retained the mutations throughout passages. By flow cytometry, we observed predominance of CD14Collectively, the results confirm that the signature mutations are restricted to stromal cells in CGCG, and the in vitro and in vivo results support that these cells have the capacity to differentiate into osteoblasts, in line with the bone formation often observed in the stroma of these lesions.
Databáze: OpenAIRE
Externí odkaz: