Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
| Autor: | Branko Bugarski, Borislav Vasić, Ivana Drvenica, Ana Stancic, Katarina Bukara, Dusan Vucetic, Radoš Gajić, Filip Kiekens, Vesna Ilić, Danijela Mišić |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Osmosis Diclofenac Erythrocytes Swine Drug Compounding Pharmaceutical biotechnology Bioengineering 02 engineering and technology Hemolysis Applied Microbiology and Biotechnology 03 medical and health sciences Drug Delivery Systems Species Specificity Amphiphile Gradual hypotonic hemolysis medicine Animals Humans Particle Size Biology Controlled drug delivery Erythrocyte membranes Chromatography Chemistry Slaughterhouse blood Pharmacology. Therapy Erythrocyte Membrane General Medicine Diclofenac Sodium 021001 nanoscience & nanotechnology medicine.disease stomatognathic diseases 030104 developmental biology Membrane Drug delivery Encapsulation Efflux 0210 nano-technology Engineering sciences. Technology Biotechnology medicine.drug |
| Zdroj: | Journal of biotechnology Journal of Biotechnology |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2016.10.017 |
| Popis: | The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process. |
| Databáze: | OpenAIRE |
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