Affinity chromatography of hepatitis B antigen
| Autor: | Hiroyuki Shiraishi, Ryoichi Shirachi, Nakao Ishida, Noriyoshi Sukeno |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
HBsAg
Chromatography Elution Sodium chemistry.chemical_element Radioimmunoassay General Medicine Chromatography Affinity General Biochemistry Genetics and Molecular Biology Hepatitis B Antigens Sepharose chemistry.chemical_compound Blood serum chemistry Affinity chromatography Humans Sodium thiocyanate |
| Zdroj: | The Tohoku Journal of Experimental Medicine. 113:393-394 |
| ISSN: | 1349-3329 0040-8727 |
| Popis: | Hepatitis B antigen (HBsAg) in human plasma was purified by affinity chromatography through a column of Sepharose 4B which had been charged with anti-HBs. Eight different elution buffers were examined for better recovery by determining the antigen titers by solid-phase radioimmunoassay (Ausria-125). Among the buffers tested, 5M NaI, 1M acetic acid (AcOH) containing 1M NaCl (pH 2.5), and 3.5 M sodium thiocyanate (NaSCN) gave good recoveries and the mast preferable result was obtained with 1M AcOH containing 1M NaCl (pH 2.5). |
| Databáze: | OpenAIRE |
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