Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis
| Autor: | D. Mooney, Robert R. Bowers, Lynn C. Thomason, Franklin W. Stahl, Richard S. Myers, Mary M. Stahl |
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| Rok vydání: | 2001 |
| Předmět: |
DNA Replication
Exonuclease Exodeoxyribonuclease V Time Factors Mutant Bacillus subtilis medicine.disease_cause Escherichia coli Genetics medicine Recombinase Molecular Biology Gene Recombination Genetic biology DNA replication DNA biology.organism_classification Bacteriophage lambda Molecular biology Rec A Recombinases Exodeoxyribonucleases Phenotype Lytic cycle Mutation biology.protein Gene Deletion Plasmids |
| Zdroj: | Molecular and General Genetics MGG. 264:716-723 |
| ISSN: | 1432-1874 0026-8925 |
| Popis: | When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage lambda. Mutants of lambda that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of lambda ea47+ in the presence of AddAB is expressed only when phage DNA replication is permitted. |
| Databáze: | OpenAIRE |
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