Bone marrow-derived mast cell differentiation is strongly reduced in histidine decarboxylase knockout, histamine-free mice
| Autor: | Zoltán Wiener, András Falus, Csaba Szalai, Márton Andrásfalvy, Péter Kovács, Andras Nagy, Éva Pállinger, Sára Tóth, Anna Erdei |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Mast cell differentiation Immunology Tryptase Biology Histidine Decarboxylase Cell Degranulation chemistry.chemical_compound Mice Bone Marrow medicine Cell Adhesion Immunology and Allergy Animals Mast Cells Cells Cultured Mice Knockout Serine Endopeptidases Degranulation Cell Differentiation General Medicine Mast cell Hematopoietic Stem Cells Histidine decarboxylase Molecular biology medicine.anatomical_structure chemistry Cell culture Antigens Surface biology.protein Female Interleukin-3 Tryptases Bone marrow Histamine |
| Zdroj: | International immunology. 14(4) |
| ISSN: | 0953-8178 |
| Popis: | Mast cells are differentiated in vitro from bone marrow precursors. In this study the development of bone marrow-derived mast cells was examined from histidine decarboxylase deficient (HDC-/-) and wild-type mice in the presence of IL-3. The number of non-adherent, tryptase- and c-kit-positive mast cells in bone marrow-derived cultures of HDC(-/-) mice was decreased compared to that of wild-type (HDC+/+) animals, but within the tryptase- and c-kit-positive cells there was no difference in the expression intensity of both markers between the two groups. Furthermore, less serine proteases mMCP5, mMCP6 and FcepsilonRIalpha mRNA were detected in bone marrow-derived cell cultures originating from HDC-/- mice. Antigen-provoked degranulation through high-affinity FcepsilonI receptor was also lower in HDC-/- mice. The colony assays in semisolid medium yielded a significantly lower ratio of mixed colonies and higher proportion of macrophage colonies from HDC-/- mice-derived bone marrow compared to the wild-type. In the course of the differentiation of HDC-/- --derived mast cells exogenously added histamine is unable to substitute the endogenously missing histamine. Concordantly, alpha-fluoromethyl-histamine, the specific inhibitor of HDC, revealed only a marginal inhibition on the differentiation of tryptase-positive mast cells from wild-type mice. These findings suggest that the effect of histamine on the IL-3-dependent development of bone marrow-derived mast cell differentiation during the early period is crucial and irreplaceable. |
| Databáze: | OpenAIRE |
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