Effect of intracellular delivery of energy metabolites on intracellular Ca2+ in mouse islets of Langerhans
| Autor: | Kerstin Nolkrantz, Gabriella M. Dahlgren, Robert T. Kennedy |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Period (gene)
Glucose-6-Phosphate Mice Inbred Strains Biology General Biochemistry Genetics and Molecular Biology Islets of Langerhans Mice Adenosine Triphosphate Fructosediphosphates Animals Glycolysis Calcium Signaling Hexosephosphates General Pharmacology Toxicology and Pharmaceutics Cells Cultured Calcium metabolism geography geography.geographical_feature_category Adenine Nucleotides Electroporation General Medicine Compartment (chemistry) Calcium Channel Blockers Islet Cell biology Adenosine Diphosphate Glucose Calcium Flux (metabolism) Intracellular |
| Zdroj: | Life Sciences. 77:2986-2997 |
| ISSN: | 0024-3205 |
| Popis: | Regulation of glucose-induced oscillations in intracellular Ca2+ concentration ([Ca2+]i) was investigated by using a novel technique, electroporation from an electrolyte-filled capillary, to deliver energy metabolites to the intracellular compartment of mouse islets. Intracellular application of ATP resulted in a nifedipine-sensitive increase in [Ca2+]i, consistent with a KATP-channel dependent mechanism of Ca2+ influx. [Ca2+]i in islets exposed to 10 mM glucose oscillated with a period of approximately 3 min, often superimposed with faster oscillations. Electroporation of ATP blocked all types of oscillations and elevated [Ca2+]i while delivery of ADP had no effect on oscillations. Intracellular delivery of glucose-6-phosphate or fructose-1,6-bisphosphate tended to transform slow oscillations to fast oscillations. These results demonstrate that modulation of ATP concentrations and glycolytic flux are important in development of slow oscillations. |
| Databáze: | OpenAIRE |
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