Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors
| Autor: | Shoji Yamaoka, Yuji Miyahara, Akira Matsumoto, Hiroaki Takeuchi, Tatsuro Goda, Yukichi Horiguchi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Influrenza virus Biomedical Engineering Biophysics Lactose Biosensing Techniques Biology medicine.disease_cause Article Chromatography Affinity Virus Microbiology Avian Influenza A Virus 03 medical and health sciences chemistry.chemical_compound Influenza A Virus H1N1 Subtype Limit of Detection Influenza Human Genotype Pandemic Electrochemistry medicine Humans Receptor Electrodes Biointerface Electrochemical Techniques General Medicine Virology Influenza A virus subtype H5N1 Sialic acid 030104 developmental biology chemistry Quartz Crystal Microbalance Techniques biology.protein Gold Molecular recognition Label-free Neuraminidase Biosensor Biotechnology |
| Zdroj: | Biosensors & Bioelectronics |
| ISSN: | 0956-5663 |
| Popis: | A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Acα(2-6)Galβ(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections. |
| Databáze: | OpenAIRE |
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