Cytotoxicity of alkylphenols and alkylated non-phenolics in a primary culture of rainbow trout (Onchorhynchus mykiss) hepatocytes
| Autor: | Eivind Farmen Finne, Knut E. Tollefsen, Camilla Blikstad, Sissel Eikvar, Inger Katharina Gregersen |
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| Rok vydání: | 2008 |
| Předmět: |
Dose-Response Relationship
Drug Molecular Structure Alkylphenol Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health General Medicine Biodegradation Pollution Hydrocarbons chemistry.chemical_compound Phenols Biochemistry chemistry Oncorhynchus mykiss Toxicity Hepatocytes Animals Ecotoxicology Organic chemistry Phenol Rainbow trout Cytotoxicity Cells Cultured Water Pollutants Chemical |
| Zdroj: | Ecotoxicology and Environmental Safety. 69:64-73 |
| ISSN: | 0147-6513 |
| Popis: | Alkylphenols are common aquatic pollutants originating from industrial use of the compounds themselves or as biodegradation products of alkylphenol polyethoxylates. The cytotoxicity of a range of alkylphenols and alkylated non-phenolics were assessed in a primary culture of rainbow trout (Onchorhynchus mykiss) hepatocytes to construct a structure-toxicity relationship for this group of ubiquitous aquatic pollutants. Metabolic inhibition and loss of membrane integrity were used as cytotoxic endpoints through use of the cellular markers Alamar blue and 5-carboxyfluorescein diacetate acetoxymethyl ester, respectively. The results show that cytotoxicity increased with the hydrophobicity of the alkylphenols for compounds with logK(OW)4.9. Normal chained alkylphenols, branched alkylphenols and multi-substituted alkylphenols with logK(OW)4.9 deviated clearly from this relationship. The alkylphenols displayed greater cytotoxicity than alkylated non-phenolics and it is proposed that most alkylated non-phenolic caused non-polar narcosis (baseline toxicity) whereas the alkylphenols caused polar narcosis. Observations that metabolic inhibition occurred at lower concentrations than loss of membrane integrity for most chemicals indicated that interference with cellular metabolic functions was the main cause of cytotoxicity. Metabolic inhibition corresponded better than loss of membrane integrity to reported acute toxicity to fish, although the in vivo acute toxicity of hydrophobic compounds (logK(OW)2-3) was clearly underestimated by both endpoints. |
| Databáze: | OpenAIRE |
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