Radioprotective effects of Sipunculus nudus L. polysaccharide combined with WR-2721, rhIL-11 and rhG-CSF on radiation-injured mice
Autor: | Wei Chen, Xianrong Shen, Liu Yuming, Dingwen Jiang, Ying He, Shuqi Jiang |
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Rok vydání: | 2015 |
Předmět: |
Male
WR-2721 Nematoda Health Toxicology and Mutagenesis Radiation-Protective Agents Spleen Biology Testicle Andrology Mice Amifostine rhIL-11 Polysaccharides White blood cell Granulocyte Colony-Stimulating Factor Sipunculus nudus medicine Animals Radiology Nuclear Medicine and imaging Radiation Injuries radioprotection Sipunculus nudus L. polysaccharide Mice Inbred ICR Radiation Interleukin-11 biology.organism_classification Recombinant Proteins Haematopoiesis Red blood cell Treatment Outcome medicine.anatomical_structure Immunology combination administration Drug Therapy Combination Bone marrow Hemoglobin rhG-CSF |
Zdroj: | Journal of Radiation Research |
ISSN: | 1349-9157 0449-3060 |
Popis: | This study investigated the radioprotective effect of Sipunculus nudus L. polysaccharide (SNP) in combination with WR-2721, rhIL-11 and rhG-CSF on irradiated mice. A total of 70 Imprinting Control Region (ICR) mice were divided into seven groups: the control group, the model group and five administration groups. All groups, except the control group, were exposed to a 5 Gy (60)Co γ-ray beam. Blood parameters [including white blood cell (WBC), red blood cell (RBC) and platelet counts and hemoglobin level] were assessed three days before irradiation, and the on the 3rd, 7th and 14th days after irradiation. Spleen, thymus and testicular indices, DNA contents of bone marrow cells, bone marrow nucleated cells, sperm counts, superoxide dismutase (SOD), malondialdehyde (MDA), testosterone and estradiol levels in the serum were assessed on the 14th day after irradiation. The combined administration of SNP, WR-2721, rhIL-11 and rhG-CSF exerted synergistic recovery effects on peripheral blood WBC, RBC and platelet counts and hemoglobin levels in irradiated mice, and synergistic promotion effects on spleen, thymus, testicle, bone marrow nucleated cells and sperm counts in irradiated mice. The synergistic administration increased the serum SOD activities and serum testosterone content of irradiated mice, but synergy decreased the content of serum MDA and estradiol in irradiated mice. These results suggest that the combined administration of SNP, WR-2721, rhIL-11 and rhG-CSF should increase the efficacy of these drugs for acute radiation sickness, protect immunity, hematopoiesis and the reproductive organs of irradiated-damaged mice, and improve oxidation resistance in the body. |
Databáze: | OpenAIRE |
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