Isolation and characterisation of T lymphocytes from sural nerve biopsies in patients with Guillain-Barre syndrome and chronic inflammatory demyelinating polyneuropathy
Autor: | P. C. Barber, J. B. Winer, Anne Ben-Smith, J. S. H. Gaston |
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Rok vydání: | 1996 |
Předmět: |
T-Lymphocytes
T cell Cell Culture Techniques Polyradiculoneuropathy Cytomegalovirus Enzyme-Linked Immunosorbent Assay Sural nerve Chronic inflammatory demyelinating polyneuropathy Campylobacter jejuni Myelin Sural Nerve Antigens CD Cell Movement medicine Humans Guillain-Barre syndrome business.industry T lymphocyte medicine.disease Psychiatry and Mental health medicine.anatomical_structure Immunology Surgery Neurology (clinical) Bacterial antigen business Polyneuropathy Research Article Demyelinating Diseases |
Zdroj: | Journal of Neurology, Neurosurgery & Psychiatry. 61:362-368 |
ISSN: | 0022-3050 |
Popis: | OBJECTIVES: To characterise cultured T lymphocytes from nerve biopsies in patients with Guillain-Barré syndrome and chronic inflammatory demyelinating polyneuropathy (CIDP). METHODS: Sural nerve biopsies, obtained from six patients with Guillain-Barré syndrome, four with CIDP, and six controls with other neuropathies, were cultured with 20 U/ml recombinant interleukin-2 (IL-2) for eight weeks. Flow cytometry was used to determine the phenotype of cultured T lymphocytes. Their proliferative responses to a range of bacterial antigens were also examined. RESULTS: T cell lines were established from four of six patients with Guillain-Barré syndrome, one of four with CIDP, one patient with peripheral nerve vasculitis, and none of five controls with non-inflammatory neuropathies. One of these T cell lines from a patient with Guillain-Barré syndrome, preceded by Campylobacter jejuni infection, consisted entirely of gamma delta TCR+ T lymphocytes. The peripheral blood of this patient also contained an increased frequency of gamma delta T cells when stimulated with C jejuni. The nerve derived T cell lines failed to show a proliferative response to bacterial antigens or to a preparation of myelin proteins. CONCLUSIONS: A new technique to isolate T cells from nerve biopsies in patients with Guillain-Barré syndrome and CIDP is reported. This technique may prove to be a useful tool in the investigation of the pathogenesis of other inflammatory neuropathies such as peripheral nerve vasculitis. The isolation of a gamma delta TCR+ nerve T cell line is of interest because of the possibility that these cells might respond to glycolipid epitopes common to C jejuni and peripheral nerve gangliosides. |
Databáze: | OpenAIRE |
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