Therapeutic effects of D-aspartate in a mouse model of multiple sclerosis
| Autor: | Laura Magliozzi, Abbas Mirshafiey, Antimo D'Aniello, Parvin Ekhtiari, Nakisa Tabrizian, Sanaz Afraei, Zahra Aghazadeh, Reza Sedaghat, Gholamreza Azizi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
antioxidant Multiple Sclerosis experimental autoimmune encephalomyelitis lcsh:TX341-641 Inflammation Neuroprotection Mice 03 medical and health sciences Myelin 0302 clinical medicine medicine Animals Interleukin 6 Pharmacology Aspartic Acid Innate immune system biology business.industry Multiple sclerosis lcsh:RM1-950 D-Aspartic Acid Experimental autoimmune encephalomyelitis D-aspartate medicine.disease Oligodendrocyte Mice Inbred C57BL lcsh:Therapeutics. Pharmacology 030104 developmental biology medicine.anatomical_structure Immunology biology.protein Female Myelin-Oligodendrocyte Glycoprotein medicine.symptom business lcsh:Nutrition. Foods and food supply 030217 neurology & neurosurgery Food Science |
| Zdroj: | Journal of Food and Drug Analysis, Vol 25, Iss 3, Pp 699-708 (2017) |
| ISSN: | 1021-9498 |
| DOI: | 10.1016/j.jfda.2016.10.025 |
| Popis: | Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis. EAE is mainly mediated by adaptive and innate immune responses that leads to an inflammatory demyelization and axonal damage. The aim of the present research was to examine the therapeutic efficacy of D-aspartic acid (D-Asp) on a mouse EAE model. EAE induction was performed in female C57BL/6 mice by myelin 40 oligodendrocyte glycoprotein (35-55) in a complete Freund's adjuvant emulsion, and D-Asp was used to test its efficiency in the reduction of EAE. During the course of study, clinical evaluation was assessed, and on Day 21, post-immunization blood samples were taken from the heart of mice for the evaluation of interleukin 6 and other chemical molecules. The mice were sacrificed, and their brain and cerebellum were removed for histological analysis. Our findings indicated that D-Asp had beneficial effects on EAE by attenuation in the severity and delay in the onset of the disease. Histological analysis showed that treatment with D-Asp can reduce inflammation. Moreover, in D-Asp-treated mice, the serum level of interleukin 6 was significantly lower than that in control animals, whereas the total antioxidant capacity was significantly higher. The data indicates that D-Asp possess neuroprotective property to prevent the onset of the multiple sclerosis. |
| Databáze: | OpenAIRE |
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