Characterization of immune response in Staphylococcus aureus chronically infected bovine mammary glands during active involution

Autor: Maria Sol Renna, Celina Baravalle, Luis Fernando Calvinho, Melisa Belen Lovato, Elizabet Amanda Lorena Pereyra, Bibiana Elisabet Dallard, Sofía Clara Sacco, Hugo Hector Ortega, Carolina Soledad Andreotti
Rok vydání: 2017
Předmět:
0301 basic medicine
S. AUREUS
Adaptive Immunity
medicine.disease_cause
0403 veterinary science
Antibody Specificity
Otras Ciencias Veterinarias
Lactation
Interleukin-1alpha
MASTITIS
Immunology and Allergy
Lymphocytes
Mastitis
Bovine
biology
Lactoferrin
Interleukin-17
Interleukin
04 agricultural and veterinary sciences
General Medicine
Staphylococcal Infections
Acquired immune system
Antibodies
Bacterial
Infectious Diseases
medicine.anatomical_structure
Staphylococcus aureus
Female
040301 veterinary sciences
Immunology
Microbiology
03 medical and health sciences
Immune system
Mammary Glands
Animal
IMMUNE RESPONSE
medicine
Animals
Involution (medicine)
General Veterinary
Tumor Necrosis Factor-alpha
Ciencias Veterinarias
Macrophages
Immunity
Innate
Toll-Like Receptor 2
TLR2
030104 developmental biology
CIENCIAS AGRÍCOLAS
biology.protein
INVOLUTION
Cattle
Zdroj: Comparative immunology, microbiology and infectious diseases. 54
ISSN: 1878-1667
Popis: The aim of this study was to characterize the immune response in Staphylococcus aureus chronicallyinfected bovine mammary glands during active involution. Twenty-one Holstein non-pregnant cows inlate lactation either uninfected or with chronic naturally acquired S. aureus intramammary infections(IMI) were included in this study. Cows were slaughtered at 7, 14 and 21 d after cessation of milkingand samples for immunohistochemical analysis were taken. Protein expression of toll-like receptor 2(TLR2) and TLR4 was significantly higher in S. aureus-infected quarters than in uninfected controls atthe three involution stages studied. Protein expression of tumor necrosis factor-alpha (TNF-á),interleukin (IL)-1á and IL-17 was significantly affected by IMI; being higher in S. aureus-infectedthan uninfected quarters during all evaluated stages. In S. aureus-infected and uninfected quartersprotein expression of lactoferrin increased from day 7 to 14 of involution, decreasing significantly today 21 in mammary quarters with chronic infections. The number of monocytes-macrophages wassignificantly higher in S. aureus-infected than in uninfected control quarters at 7 and 21 d ofinvolution. The number of T lymphocytes was significantly higher in S. aureus-infected than inuninfected quarters at 7 and 14 d of involution while the number of B lymphocytes was significantlyhigher in S. aureus-infected than in uninfected quarters during all evaluated stages, showing aprogressive increase as involution advanced. These results demonstrated a sustained and exacerbatedinnate and adaptive immune response during chronic S. aureus IMI, playing a critical role in theinfection control during active involution Fil: Andreotti, Carolina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Baravalle, Celina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Sacco, Sofía Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Lovato, Melisa Belen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Pereyra, Elizabet Amanda Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ortega, Hugo Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Clínicas; Argentina Fil: Dallard, Bibiana Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Databáze: OpenAIRE
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