Geranylgeraniol-induced Myogenic Differentiation of C2C12 Cells
| Autor: | Tsuyoshi Nakajima, Mari Fukuzaki, Shoichiro Kokabu, Yoshiyuki Yoshimoto, Yukiko Takahashi, Ryo Masuda, Min Zhang, Takuma Matsubara, Katsura Saeki, Mariko Urata, Kazmasa Morikawa, Chihiro Nakatomi, William N. Addison, Atsuko Nakamichi |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Geranylgeranyl pyrophosphate General Biochemistry Genetics and Molecular Biology Cell Line Myoblasts 03 medical and health sciences chemistry.chemical_compound Mice Geranylgeranylation Geranylgeraniol medicine Myocyte Animals Cell Proliferation Pharmacology Myogenesis Skeletal muscle Cell Differentiation musculoskeletal system Immunohistochemistry Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Mevalonate pathway Diterpenes C2C12 tissues Metabolic Networks and Pathways Research Article |
| Popis: | Background Geranylgeraniol (GGOH) is a C20 isoprenoid found in fruits, vegetables, and grains, including rice. As a food substance, GGOH is categorized as 'Generally Recognized as Safe'. GGOH is an intermediate product in the mevalonate pathway and acts as a precursor to geranylgeranyl pyrophosphate. Materials and methods C2C12 mouse myoblasts derived from muscle satellite cells were used. Quantitative reverse-transcriptase polymerase chain reaction, western blotting analysis, and immunocytochemical analysis were performed to respectively assess mRNA expression, protein levels, and the number of myofibers. Results GGOH reduced the expression levels of skeletal muscle atrophy-related ubiquitin ligases in myofibers derived from C2C12 cells. GGOH induced myogenic differentiation of C2C12 cells via geranylgeranylation. GGOH did not adversely affect the proliferation of C2C12 cells. Conclusion GGOH induces myoblast differentiation in C2C12 cells. |
| Databáze: | OpenAIRE |
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