Novel expression and regulation of the renin-angiotensin system in metanephric organ culture
| Autor: | Robert M. Carey, Victoria F. Norwood, M. Garmey, R. Ariel Gomez, Jeffrey Wolford |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Angiotensin receptor Physiology Kidney development Biology Organ culture Kidney Receptor Angiotensin Type 2 Receptor Angiotensin Type 1 Rats Sprague-Dawley Renin-Angiotensin System Organ Culture Techniques Physiology (medical) Internal medicine Renin–angiotensin system Renin medicine Animals RNA Messenger In Situ Hybridization Receptors Angiotensin Reverse Transcriptase Polymerase Chain Reaction Angiotensin II Embryogenesis Embryo Mammalian Embryonic stem cell Immunohistochemistry Rats Endocrinology medicine.anatomical_structure |
| Zdroj: | American journal of physiology. Regulatory, integrative and comparative physiology. 279(2) |
| ISSN: | 0363-6119 |
| Popis: | To evaluate the presence and regulation of the renin-angiotensin system (RAS) in metanephric organ culture, embryonic day 14 (E14) rat metanephroi were cultured for 6 days. mRNAs for renin and both ANG II receptors (AT1and AT2) are expressed at E14, and all three genes continue to be expressed in culture. Renin mRNA is localized to developing tubules and ureteral branches in the cultured explants. At E14, renin immunostaining is found in isolated cells scattered within the mesenchyme. As differentiation progresses, renin localizes to the ureteric epithelium, developing tubules and glomeruli. E14 metanephroi contain ANG II, and peptide production persists in culture. Renin activity is present at E14 (6.13 ± 0.61 pg ANG I · kidney−1· h−1) and in cultured explants (28.84 ± 1.13 pg ANG I · kidney−1· h−1). Renin activity in explants is increased by ANG II treatment (70.1 ± 6.36 vs. 40.97 ± 1.94 pg ANG I · kidney−1· h−1in control). This increase is prevented by AT1blockade, whereas AT2antagonism has no effect. These studies document an operational local RAS and a previously undescribed positive-feedback mechanism for renin generation in avascular, cultured developing metanephroi. This novel expression pattern and regulatory mechanism highlight the unique ability of developing renal cells to express an active RAS. |
| Databáze: | OpenAIRE |
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