Simultaneous determination of cations, zwitterions and neutral compounds using mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography
| Autor: | Jingyi Li, Shan Shao, Markian S. Jaworsky, Paul Kurtulik |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Chromatography
Chemistry Elution Organic Chemistry Ion chromatography Reproducibility of Results General Medicine Reversed-phase chromatography Chromatography Ion Exchange Sensitivity and Specificity Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Mixed-mode chromatography Cations Zwitterion Cation Exchange Resins Organic Chemicals Ionic compound Drug Contamination Acetonitrile Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 1185:185-193 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2008.01.083 |
| Popis: | A novel mixed-mode reversed-phase and cation-exchange high-performance liquid chromatography (HPLC) method is described to simultaneously determine four related impurities of cations, zwitterions and neutral compounds in developmental Drug A. The commercial column is Primesep 200 containing hydrophobic alkyl chains with embedded acidic groups in H(+) form on a silica support. The mobile phase variables of acid additives, contents of acetonitrile and concentrations of potassium chloride have been thoroughly investigated to optimize the separation. The retention factors as a function of the concentrations of potassium chloride and the percentages of acetonitrile in the mobile phases are investigated to get an insight into the retention and separation mechanisms of each related impurity and Drug A. Furthermore, the elution orders of the related impurities and Drug A in an ion-pair chromatography (IPC) are compared to those in the mixed-mode HPLC to further understand the chromatographic retention behaviors of each related impurity and Drug A. The study found that the positively charged Degradant 1, Degradant 2 and Drug A were retained by both ion-exchange and reversed-phase partitioning mechanisms. RI2, a small ionic compound, was primarily retained by ion-exchange. RI4, a neutral compound, was retained through reversed-phase partitioning without ion-exchange. Moreover, the method performance characteristics of selectivity, sensitivity and accuracy have been demonstrated to be suitable to determine the related impurities in the capsules of Drug A. |
| Databáze: | OpenAIRE |
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