Multi-matrix assay of cortisol, cortisone and corticosterone using a combined MEPS-HPLC procedure

Autor: Maria Addolorata Saracino, Nadia Ghedini, Maria Augusta Raggi, Corrado Iacono, Lorenzo Somaini, Gilberto Gerra
Přispěvatelé: Maria A. Saracino, Corrado Iacono, Lorenzo Somaini, Gilberto Gerra, Nadia Ghedini, Maria A. Raggi
Rok vydání: 2014
Předmět:
Hydrocortisone
Clinical Biochemistry
Pharmaceutical Science
Adrenal Cortex Hormone
Solid-phase microextraction
High-performance liquid chromatography
Analytical Chemistry
Dried blood and urine spot
Plasma
Adrenal Cortex Hormones
Limit of Detection
Drug Discovery
Corticosteroid
Chromatography
High Pressure Liquid
Spectroscopy
medicine.diagnostic_test
Heroin Dependence
Chemistry
Healthy Volunteer
Healthy Volunteers
Analgesics
Opioid
Substance Abuse Detection
Spectrophotometry
Microextraction by packed sorbent
Buffer
Human
medicine.drug
Analyte
Bioanalysis
Acetonitriles
Sorbent
Reproducibility of Result
Phosphate
Buffers
Phosphates
medicine
Humans
Acetonitrile
Saliva
Solid Phase Microextraction
Detection limit
Chromatography
Drug Discovery3003 Pharmaceutical Science
Reproducibility of Results
Cortisone
Immunoassay
Adsorption
Corticosterone
Zdroj: Journal of Pharmaceutical and Biomedical Analysis. 88:643-648
ISSN: 0731-7085
Popis: The development and validation of a bioanalytical assay for the simultaneous determination of cortisol, cortisone and corticosterone levels in several matrices, such as saliva, plasma, blood and urine samples have been described. The method is based on a rapid test which combines a microextraction by packed sorbent procedure and liquid chromatography-diode array technique. Chromatographic separation of the analytes (cortisol, cortisone and corticosterone) and the internal standard (methylprednisolone) was achieved in less than 10 min on a reversed-phase pentafluorophenyl column using a mobile phase com-posed of phosphate buffer and acetonitrile. The assay was performed after an innovative microextraction procedure by means of C8 sorbent which guaranteed good clean-up of the matrices and satisfactory extraction yield of the analytes. Moreover, the method gave linear results over a range of 5–100 ng mL−1 and showed good selectivity and precision. This method was successfully applied for quantifying corticosteroids in specific matrices derived from some healthy volunteers in comparison to two socially diversified groups, namely former heroin addicts undergoing opioid replacement therapy and poly-drug abusers The development and validation of a bioanalytical assay for the simultaneous determination of cortisol, cortisone and corticosterone levels in several matrices, such as saliva, plasma, blood and urine samples have been described. The method is based on a rapid test which combines a microextraction by packed sorbent procedure and liquid chromatography-diode array technique. Chromatographic separation of the analytes (cortisol, cortisone and corticosterone) and the internal standard (methylprednisolone) was achieved in less than 10min on a reversed-phase pentafluorophenyl column using a mobile phase composed of phosphate buffer and acetonitrile. The assay was performed after an innovative microextraction procedure by means of C8 sorbent which guaranteed good clean-up of the matrices and satisfactory extraction yield of the analytes. Moreover, the method gave linear results over a range of 5-100ngmL-1 and showed good selectivity and precision.This method was successfully applied for quantifying corticosteroids in specific matrices derived from some healthy volunteers in comparison to two socially diversified groups, namely former heroin addicts undergoing opioid replacement therapy and poly-drug abusers. © 2013 Elsevier B.V.
Databáze: OpenAIRE
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