Loss of Snf5 Induces Formation of an Aberrant SWI/SNF Complex
| Autor: | Mekonnen Lemma Dechassa, Arjan Hada, Solomon G. Hailu, Blaine Bartholomew, Sandipan Brahma, Payel Sen, Jie Luo, Jeff Ranish, Somnath Paul, Jim Persinger |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Chromosomal Proteins Non-Histone cells genetic processes Gene Expression macromolecular substances General Biochemistry Genetics and Molecular Biology Chromatin remodeling Article Fungal Proteins 03 medical and health sciences Yeasts Nucleosome Chromatin structure remodeling (RSC) complex SMARCB1 lcsh:QH301-705.5 Adenosine Triphosphatases Cell Nucleus Nucleosome binding biology SWI/SNF complex Chemistry Chromatin Assembly and Disassembly SWI/SNF Cell biology Nucleosomes DNA-Binding Proteins enzymes and coenzymes (carbohydrates) Protein Subunits 030104 developmental biology lcsh:Biology (General) health occupations SMARCA4 biology.protein biological phenomena cell phenomena and immunity Transcription Factors |
| Zdroj: | Cell Reports, Vol 18, Iss 9, Pp 2135-2147 (2017) |
| ISSN: | 2211-1247 |
| Popis: | Summary: The SWI/SNF chromatin remodeling complex is highly conserved from yeast to human, and aberrant SWI/SNF complexes contribute to human disease. The Snf5/SMARCB1/INI1 subunit of SWI/SNF is a tumor suppressor frequently lost in pediatric rhabdoid cancers. We examined the effects of Snf5 loss on the composition, nucleosome binding, recruitment, and remodeling activities of yeast SWI/SNF. The Snf5 subunit is shown by crosslinking-mass spectrometry (CX-MS) and subunit deletion analysis to interact with the ATPase domain of Snf2 and to form a submodule consisting of Snf5, Swp82, and Taf14. Snf5 promotes binding of the Snf2 ATPase domain to nucleosomal DNA and enhances the catalytic and nucleosome remodeling activities of SWI/SNF. Snf5 is also required for SWI/SNF recruitment by acidic transcription factors. RNA-seq analysis suggests that both the recruitment and remodeling functions of Snf5 are required in vivo for SWI/SNF regulation of gene expression. Thus, loss of SNF5 alters the structure and function of SWI/SNF. : Mutation of SWI/SNF chromatin remodeling complex subunits contributes to cancer and neurological disorders. Sen et al. report that loss of the Snf5 subunit alters the architecture and function of SWI/SNF in a yeast model system. These findings may reflect changes that occur in pediatric rhabdoid tumors with mutated Snf5. Keywords: Chromatin remodeling, SWI/SNF, Snf5, SMARCB1, BAF47, INI1 |
| Databáze: | OpenAIRE |
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