Fibrin Glue Implants Seeded with Dental Pulp and Periodontal Ligament Stem Cells for the Repair of Periodontal Bone Defects: A Preclinical Study
| Autor: | N. I. Enukashvily, Tatiana Paderina, Anastasia V Kotova, Roman E Banashkov, S. S. Bilyk, Alexey V Silin, Natalia Semenova, Anton N. Kovalenko, Irina Karabak, Julia A Dombrovskaya, Dmitry Baram, Egor M Prikhodko, Dmitry Ivolgin, Wolf-Dieter Grimm |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Technology
Materials science Periodontal ligament stem cells QH301-705.5 Bioengineering Bone healing scaffold Bone tissue Fibrin Article 03 medical and health sciences 0302 clinical medicine cell technologies in regenerative dentistry Dental pulp stem cells medicine Biology (General) Fibrin glue Dental alveolus 030304 developmental biology 3D printed scaffold mold bone defect 0303 health sciences biology computed tomography 030206 dentistry dental pulp stem cells fibrin glue medicine.anatomical_structure biology.protein Implant Biomedical engineering |
| Zdroj: | Bioengineering, Vol 8, Iss 75, p 75 (2021) Bioengineering Volume 8 Issue 6 |
| ISSN: | 2306-5354 |
| Popis: | A technology to create a cell-seeded fibrin-based implant matching the size and shape of bone defect is required to create an anatomical implant. The aim of the study was to develop a technology of cell-seeded fibrin gel implant creation that has the same shape and size as the bone defect at the site of implantation. Using computed tomography (CT) images, molds representing bone defects were created by 3D printing. The form was filled with fibrin glue and human dental pulp stem cells (DPSC). The viability, set of surface markers and osteogenic differentiation of DPSC grown in fibrin gel along with the clot retraction time were evaluated. In mice, an alveolar bone defect was created. The defect was filled with fibrin gel seeded with mouse DPSC. After 28 days, the bone repair was analyzed with cone beam CT and by histological examination. The proliferation rate, set of surface antigens and osteogenic potential of cells grown inside the scaffold and in 2D conditions did not differ. In mice, both cell-free and mouse DPSC-seeded implants increased the bone tissue volume and vascularization. In mice with cell-seeded gel implants, the bone remodeling process was more prominent than in animals with a cell-free implant. The technology of 3D-printed forms for molding implants can be used to prepare implants using components that are not suitable for 3D printing. |
| Databáze: | OpenAIRE |
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