miR‑34c‑5p targets Notch1 and suppresses the metastasis and invasion of cervical cancer
| Autor: | Xiaolan Wang, Xiumin Niu, Xiaojuan Li, Ruili Jiao, Huali Wei, Sumei Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
0301 basic medicine Cancer Research cervical cancer proliferation Cell Uterine Cervical Neoplasms Biology Biochemistry Metastasis HeLa 03 medical and health sciences microRNA-34c-5p 0302 clinical medicine microRNA Genetics medicine Humans Neoplasm Invasiveness RNA Neoplasm Neoplasm Metastasis Receptor Notch1 Molecular Biology Aged Oncogene Cancer Articles Middle Aged Cell cycle medicine.disease biology.organism_classification migration and invasion Neoplasm Proteins MicroRNAs 030104 developmental biology medicine.anatomical_structure Oncology Apoptosis 030220 oncology & carcinogenesis Cancer research Molecular Medicine Female Notch homolog 1 HeLa Cells |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Micro (mi)RNAs are crucial participants in the progression of cervical cancer (CC). Growing evidence indicates that miRNA (miR)‑34c‑5p is a pivotal tumor suppressor in numerous types of cancer and its functions in CC require further investigating. The present study demonstrated that there was a decreased level of miR‑34c‑5p in CC‑associated cell lines compared with healthy control samples. It also demonstrated that miR‑34c‑5p targeted Notch1 and suppressed CC progression. Dual‑luciferase reporter assays verified the targeted relationship of miR‑34c‑5p and Notch1. The expression of Notch1 in HeLa cells was markedly reduced following miR‑34c‑5p overexpression and the proliferation, migration and invasion of HeLa cells were reduced although apoptosis was accelerated. However, overexpression of miR‑34c‑5p was reversed following the addition of Notch1, which supported the finding of the targeted relationship between miR‑34c‑5p and Notch1. Flow cytometry demonstrated that miR‑34c‑5p inhibited the proliferation of HeLa cells while accelerating apoptosis. The present study concluded that miR‑34c‑5p was a tumor suppressor in CC and may be a novel measure for the future treatment of CC. |
| Databáze: | OpenAIRE |
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