FGF-20, a Novel Neurotrophic Factor, Preferentially Expressed in the Substantia Nigra Pars Compacta of Rat Brain

Autor: Nobuyuki Itoh, Tomokazu Ibi, Tadahisa Mikami, Shigeki Ohmachi, Naoki Kusu, Akinori Akaike, Yohko Watanabe
Rok vydání: 2000
Předmět:
Fibroblast Growth Factor 9
DNA
Complementary
Insecta
Cell Survival
Dopamine
Molecular Sequence Data
Biophysics
Glutamic Acid
Substantia nigra
In situ hybridization
Biology
Transfection
Biochemistry
Cell Line
law.invention
Evolution
Molecular
Midbrain
law
Neurotrophic factors
Animals
Humans
Tissue Distribution
Amino Acid Sequence
Rats
Wistar
Growth Substances
Molecular Biology
Peptide sequence
Cells
Cultured
In Situ Hybridization
Phylogeny
chemistry.chemical_classification
Dose-Response Relationship
Drug
Sequence Homology
Amino Acid
Reverse Transcriptase Polymerase Chain Reaction
Pars compacta
Brain
Chromosome Mapping
Cell Biology
Anatomy
Molecular biology
Recombinant Proteins
Rats
Amino acid
Fibroblast Growth Factors
Substantia Nigra
chemistry
Recombinant DNA
Baculoviridae
Chromosomes
Human
Pair 8
Zdroj: Biochemical and Biophysical Research Communications. 277:355-360
ISSN: 0006-291X
DOI: 10.1006/bbrc.2000.3675
Popis: We have isolated cDNA encoding a novel FGF (212 amino acids) from rat brain. Because this is the 20th documented member of the FGF family, we tentatively term it FGF-20. Among FGF family members, FGF-20 is most similar to FGF-9 and FGF-16 (70 and 62% amino acid identity, respectively). Human FGF-20 gene was found in the human genomic sequence mapped to the 8p21.3-p22 region. Human FGF-20 is highly identical to rat FGF-20 (95% amino acid identity). FGF-20 mRNA was preferentially expressed in rat brain among the adult major tissues examined. The localization of FGF-20 mRNA in rat brain was also examined by in situ hybridization. FGF-20 mRNA was preferentially expressed in the substantia nigra pars compacta. To examine the biological activity of FGF-20, recombinant rat FGF-20 was produced by insect cells infected with recombinant baculovirus containing rat FGF-20 cDNA. Recombinant rat FGF-20 enhanced the survival of midbrain dopaminergic neurons. The present results indicate that FGF-20 is a novel neurotrophic factor preferentially expressed in the substantia nigra pars compacta of rat brain.
Databáze: OpenAIRE
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