Mutated c-K-ras in Small Pancreatic Adenocarcinomas
| Autor: | Jonathan L. Myles, Steven Glasner, Elna T. Kuhlmann, Barbara K. Schaeffer, Daniel S. Longnecker |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pancreatic disease
Endocrinology Diabetes and Metabolism Molecular Sequence Data Adenocarcinoma Biology medicine.disease_cause law.invention Pathogenesis Endocrinology law Internal Medicine medicine Humans Gene Polymerase chain reaction Mutation Base Sequence Hepatology medicine.disease Pancreatic Neoplasms Genes ras medicine.anatomical_structure Tissue sections Cancer research Pancreas |
| Zdroj: | Pancreas. 9:161-165 |
| ISSN: | 0885-3177 |
| DOI: | 10.1097/00006676-199403000-00004 |
| Popis: | The objective of this study was to determine whether small human pancreatic adenocarcinomas contain activated c-K-ras as an approach to answering the question of whether c-K-ras mutation is an early change in this disease. Eight pancreatic adenocarcinomas in the range 1.2-3 cm were analyzed for c-K-ras mutation at codon 12 by amplifying the c-K-ras gene around codon 12 out of paraffin-embedded tissue sections using the polymerase chain reaction. c-K-ras mutations were detected by allele-specific oligonucleotide hybridization. Six of the eight small pancreatic adenocarcinomas contained mutated c-K-ras at codon 12, position 2, and two of the six tumors had an additional mutation at position 1 of codon 12. Our results indicate that small pancreatic adenocarcinomas are similar to large, late-stage pancreatic adenocarcinomas in that 75% of the tumors analyzed contain mutated c-K-ras at codon 12, position 2. These data suggest that c-K-ras mutation occurs early and may therefore have a role in initiation of human pancreatic adenocarcinoma. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|