Dissociation of oxidized 80-S ribosomes by sodium deoxycholate
| Autor: | James S. Clegg, A.L. Golub |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Time Factors
Chemical Phenomena Ultraviolet Rays Phenylalanine Sodium Detergents chemistry.chemical_element Biochemistry Genetics and Molecular Biology (miscellaneous) Ribosome Bile Acids and Salts Saccharomyces chemistry.chemical_compound Crustacea Polysome Centrifugation Density Gradient Animals Magnesium Centrifugation Sodium dodecyl sulfate Sodium Cholate Hydrogen peroxide Chromatography Spectrum Analysis Proteins Hydrogen Peroxide Cold Temperature Chemistry chemistry RNA Oxidation-Reduction Ribosomes Echinodermata |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 182:121-134 |
| ISSN: | 0005-2787 |
| DOI: | 10.1016/0005-2787(69)90527-9 |
| Popis: | 1. 80-S Ribosomes obtained from brine-shrimp gastrulae, sea-urchin eggs, and yeast were readily dissociated into two major subunits by sequential treatment with millimolar quantities of NaOCl or H 2 O 2 , and 0.5% sodium deoxycholate. 2. The ability of deoxycholate to dissociate NaOCl-treated ribosomes was shared by two other anionic detergents, sodium dodecyl sulfate and sodium dodecyl sarcosinate. Several nonionic detergents were tested but none produced dissociations. Anionic deoxycholate analogues, sodium cholate for example, were likewise ineffective. 3. The extent of ribosome dissociation produced by deoxycholate was a function of the concentration of NaOCl and deoxycholate used, and the temperature and duration of incubation. Although elevated Mg 2+ concentrations prevented dissociation, the effect of the detergent could not be attributed simply to the removal of bulk Mg 2+ from the incubation medium. 4. Treatment of ribosomes with NaOCl alone did not alter their ultraviolet-absorption spectra, density gradient sedimentation profiles, or RNA and protein content when compared with controls, indicating that major changes in ribosome composition were not produced by the oxidizing agent. However, poly U-directed phenylalanine incorporation by 80-S ribosomes was greatly reduced after NaOCl treatment, suggesting that groups on the ribosome associated with poly U attachment or function were among those affected by the oxidation. 5. Polyribosomes and “loaded” monosomes were not degraded by NaOCl-deoxycholate treatment. |
| Databáze: | OpenAIRE |
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