Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells
| Autor: | Onder Alpdogan, Robert M. Lavker, J. Mario Wolosin, M.T. Budak, Mingyuan Zhou, M. A. Murat Akinci |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Cellular differentiation
Cell Separation Biology Limbus Corneae Article Flow cytometry Colony-Forming Units Assay Antigen Side population medicine ATP Binding Cassette Transporter Subfamily G Member 2 Animals Humans Cell Lineage Enzyme Inhibitors Clonogenic assay Cells Cultured Corneal epithelium Cell Proliferation medicine.diagnostic_test Cell growth Stem Cells Cell Differentiation Epithelial Cells Cell Biology Flow Cytometry Immunohistochemistry Cell biology Neoplasm Proteins medicine.anatomical_structure Phenotype ATP-Binding Cassette Transporters Benzimidazoles sense organs Rabbits Stem cell Conjunctiva Biomarkers |
| Popis: | When cell populations are incubated with the DNA-binding dye Hoechst 33342 and subjected to flow cytometry analysis for Hoechst 33342 emissions, active efflux of the dye by the ABCG2/BCRP1 transporter causes certain cells to appear as a segregated cohort, known as a side population (SP). Stem cells from several tissues have been shown to possess the SP phenotype. As the lack of specific surface markers has hindered the isolation and subsequent biochemical characterization of epithelial stem cells this study sought to determine the existence of SP cells and expression of ABCG2 in the epithelia of the ocular surface and evaluate whether such SP cells had features associated with epithelial stem cells. Human and rabbit limbal-corneal and conjunctival epithelial cells were incubated with Hoechst 33342, and analyzed and sorted by flow cytometry. Sorted cells were subjected to several tests to determine whether the isolated SP cells displayed features consistent with the stem cell phenotype. Side populations amounting to |
| Databáze: | OpenAIRE |
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