Different effects of carbon ion and gamma-irradiation on expression of receptor activator of NF-kB ligand in MC3T3-E1 osteoblast cells
| Autor: | Jun-etsu Mizoe, Masahiko Sawajiri, Masayuki Okazaki, Ryo Nishikiori, Ujjal K. Bhawal, Yuji Nomura, Keiji Tanimoto |
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| Rok vydání: | 2007 |
| Předmět: |
musculoskeletal diseases
medicine.medical_specialty Acid Phosphatase Gene Expression Bone Marrow Cells General Biochemistry Genetics and Molecular Biology Cell Line Mice Osteoprotegerin Osteoclast Internal medicine medicine Animals Carbon Radioisotopes Tartrate-resistant acid phosphatase Osteoblasts biology Chemistry Tartrate-Resistant Acid Phosphatase RANK Ligand Acid phosphatase NF-kappa B Osteoblast General Medicine Molecular biology Coculture Techniques Isoenzymes Endocrinology medicine.anatomical_structure Cell culture RANKL Gamma Rays biology.protein Bone marrow |
| Zdroj: | Bulletin of experimental biology and medicine. 142(5) |
| ISSN: | 0007-4888 |
| Popis: | We investigated the effects of carbon ion and gamma-irradiation on osteoblastic MC3T3-E1 cells by comparing mRNA expression levels for RANKL and osteoprotegerin by RT-PCR. MC3T3-E1 cells were irradiated with 2, 4, or 6 Gy of carbon ions or gamma-rays, and total RNA was harvested 1, 2, 3, 5, or 7 days after irradiation. The RANKL mRNA/OPG mRNA ratio in carbon ion-irradiated MC3T3-E1 cells was lower, while in gamma-irradiated MC3T3-E1 cells this ratio was higher than in non-irradiated cells. To evaluate osteoclastogenesis of MC3T3-E1 cells, carbon ion- or gamma-irradiated cells were co-cultured with non-irradiated cells from murine bone marrow. Staining for tartrate-resistant acid phosphatase (TRAP) in co-cultures showed that carbon ion irradiation suppressed osteoclastogenesis. This result is consistent with the lower RANKL/OPG mRNA ratio for carbon ion-irradiated cells. These results suggest that carbon ion irradiation acts primarily on osteoblastic cells, leading to a decrease in the RANKL/OPG mRNA ratio. This effect, in turn, leads to a decrease in osteoclastogenesis and osteoclast activity, which results in an increase in bone volume. |
| Databáze: | OpenAIRE |
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