Salidroside promoted osteogenic differentiation of adipose-derived stromal cells through Wnt/β-catenin signaling pathway
| Autor: | Fu-ling Chen, Hong-lin Shen, Xiao-hua Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Small interfering RNA Stromal cell Wnt/β-catenin signaling pathway Cell Diseases of the musculoskeletal system 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Western blot Glucosides Phenols Osteogenesis Osteogenic differentiation Medicine Humans Orthopedics and Sports Medicine Viability assay Wnt Signaling Pathway Adipose-derived stromal cells Cells Cultured beta Catenin Orthopedic surgery 030222 orthopedics medicine.diagnostic_test business.industry Salidroside Wnt signaling pathway Cell Differentiation Cell biology RUNX2 030104 developmental biology medicine.anatomical_structure chemistry Adipose Tissue RC925-935 Surgery Stromal Cells business RD701-811 Research Article |
| Zdroj: | Journal of Orthopaedic Surgery and Research, Vol 16, Iss 1, Pp 1-9 (2021) Journal of Orthopaedic Surgery and Research |
| Popis: | Background Bone disease causes short-term or long-term physical pain and disability. It is necessary to explore new drug for bone-related disease. This study aimed to explore the role and mechanism of Salidroside in promoting osteogenic differentiation of adipose-derived stromal cells (ADSCs). Methods ADSCs were isolated and treated with different dose of Salidroside. Cell count kit-8 (CCK-8) assay was performed to assess the cell viability of ADSCs. Then, ALP and ARS staining were conducted to assess the early and late osteogenic capacity of ADSCs, respectively. Then, differentially expressed genes were obtained by R software. Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the differentially expressed genes were further analyzed. The expression of OCN, COL1A1, RUNX2, WNT3A, and β-catenin were measured by real-time PCR and Western blot analysis. Last, β-catenin was silenced by small interfering RNA. Results Salidroside significantly increased the ADSCs viability at a dose-response manner. Moreover, Salidroside enhanced osteogenic capacity of ADSCs, which are identified by enhanced ALP activity and calcium deposition. A total of 543 differentially expressed genes were identified between normal and Salidroside-treated ADSCs. Among these differentially expressed genes, 345 genes were upregulated and 198 genes were downregulated. Differentially expressed genes enriched in the Wnt/β-catenin signaling pathway. Western blot assay indicated that Salidroside enhanced the WNT3A and β-catenin expression. Silencing β-catenin partially reversed the promotion effects of Salidroside. PCR and Western blot results further confirmed these results. Conclusion Salidroside promoted osteogenic differentiation of ADSCs through Wnt/β-catenin signaling pathway. |
| Databáze: | OpenAIRE |
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