Interferon-beta induces a long-lasting antiviral state in human respiratory epithelial cells
| Autor: | Giel R. Gaajetaan, Gernot Rohde, Frank R. M. Stassen, Cathrien A. Bruggeman, Niki L. Reynaert, Juanita H. J. Vernooy, Erik V. Beuken, Gert Grauls, Mieke A. Dentener, Tanja H. Geelen |
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| Přispěvatelé: | Pulmonologie, Medische Microbiologie, RS: NUTRIM - R3 - Chronic inflammatory disease and wasting, RS: CAPHRI School for Public Health and Primary Care |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Cell type Rhinovirus INTRANASAL INTERFERON-ALPHA-2 VIRUSES COLDS Respiratory Mucosa Biology medicine.disease_cause Antiviral Agents OBSTRUCTIVE PULMONARY-DISEASE Cell Line Interferon medicine Humans COPD A549 cells Respiratory system A549 cell Primary bronchial epithelial cells Dose-Response Relationship Drug RHINOVIRUS INFECTIONS VIRAL-INFECTIONS Epithelial Cells Interferon-beta ASTHMA EXACERBATIONS medicine.disease Interferon beta ISG15 Virology Asthma ALPHA Infectious Diseases Gene Expression Regulation Immunology Respiratory epithelium medicine.drug |
| Zdroj: | Journal of Infection, 66(2), 163-169. Elsevier Saunders |
| ISSN: | 0163-4453 |
| Popis: | OBJECTIVES: Interferon-beta (IFNbeta) induces strong antiviral effects and is therefore an attractive agent to prevent or reduce the incidence of virus-mediated exacerbations in asthmatic or chronic obstructive pulmonary disease (COPD) patients. We therefore investigated the effects of prophylactic IFNbeta on respiratory epithelial cells infected with rhinovirus (RV). METHODS: A549 cells and primary bronchial epithelial cells (PBECs) were exposed for 18 h to IFNbeta. Then, IFNbeta was either removed or maintained in the supernatant for the rest of the experiment and cells were infected with RV-1B at t = 0 or 72 h after the initial exposure to IFNbeta. RESULTS: Viral RNA levels were decreased in both cell types. Furthermore, both viral RNA and infectious virus levels in the supernatant of infected A549 cells were still significantly reduced at 72 h after removal of IFNbeta. This pronounced antiviral pre-treatment effect was associated with increased expression of the antiviral genes IFN-stimulated protein of MR15000 (ISG15) and Myxovirus resistance 1 (Mx1) and the effect was maintained even when IFNbeta levels in the supernatant of A549 cells were undetectable. CONCLUSIONS: These data show that IFNbeta has not only a strong, but also a long-lasting protective effect against RV infection of respiratory epithelium. |
| Databáze: | OpenAIRE |
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