Visualisation of biopolymer mixtures using confocal scanning laser microscopy (CSLM) and covalent labelling techniques
| Autor: | Marijke W. Edelman, Erik van der Linden, R. Hans Tromp, Fred van de Velde, Fanny Weinbreck |
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| Rok vydání: | 2003 |
| Předmět: |
Physics and Physical Chemistry of Foods
food.ingredient Starch Confocal whey engineering.material Polysaccharide chemistry.chemical_compound Colloid and Surface Chemistry food Labelling Microscopy Physical and Theoretical Chemistry VLAG chemistry.chemical_classification light-microscopy Chromatography food and beverages Surfaces and Interfaces General Medicine phase-separation proteins Gellan gum chemistry engineering Gum arabic Biopolymer Biotechnology |
| Zdroj: | Colloids and Surfaces. B: Biointerfaces 31 (2003) 1-4 Colloids and Surfaces. B: Biointerfaces, 31(1-4), 159-168 |
| ISSN: | 0927-7765 |
| Popis: | Confocal scanning laser microscopy (CSLM) has been used to study the behaviour of mixtures of proteins, gelatine, whey proteins and β-lactoglobulin, and polysaccharides, dextran, gellan gum, carrageenan, gum Arabic, and starch. CSLM proved to be a suitable technique to visualise the microstructure of these (phase separated) mixtures in two and three-dimensional images. Contrast through fluorescence is obtained either by covalent labelling (polysaccharides and proteins) or non-covalent labelling (proteins and starch). Double and triple labelling allows the visualisation of individual components in a complex mixture of biopolymers. |
| Databáze: | OpenAIRE |
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