Neuropeptide Y upregulates Runx2 and osterix and enhances osteogenesis in mouse MC3T3-E1 cells via an autocrine mechanism

Autor: Chunzheng Gao, Yuan Chen, Bo Zhang, Xiaolei Zhang, Xuguang Zhou, Juan Xiao
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
musculoskeletal diseases
Cancer Research
Small interfering RNA
neuropeptide Y
MC3T3-E1 cell line
Cellular differentiation
Osteocalcin
Core Binding Factor Alpha 1 Subunit
runt-related transcription factor 2
Biochemistry
Cell Line
03 medical and health sciences
Mice
0302 clinical medicine
Downregulation and upregulation
stomatognathic system
Osteogenesis
mental disorders
Genetics
medicine
Animals
RNA
Small Interfering
Autocrine signalling
Molecular Biology
Osteoblasts
biology
Chemistry
musculoskeletal
neural
and ocular physiology
Osteoblast
Cell Differentiation
Articles
Neuropeptide Y receptor
Alkaline Phosphatase
small interfering RNA
humanities
Cell biology
Up-Regulation
RUNX2
Autocrine Communication
030104 developmental biology
medicine.anatomical_structure
Oncology
Sp7 Transcription Factor
030220 oncology & carcinogenesis
osterix
biology.protein
Molecular Medicine
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
Popis: The neuropeptide Y (NPY) system is considered one of the primary neural signaling pathways. NPY, produced by osteoblasts and other peripheral tissues, is known to inhibit biological functions of osteoblasts. However, until recently, little was known of the autocrine mechanism by which NPY is regulated. To investigate this mechanism, overexpression plasmids and small interfering RNA (siRNA) targeting NPY were transfected into the MC3T3‑E1 cell line to observe its effects on osteogenesis. NPY overexpression was found to markedly enhance the osteogenic ability of MC3T3‑E1 cells by an autocrine mechanism, coincident with the upregulation of osterix and runt‑related transcription factor 2 (Runx2). Furthermore, NPY increased the activities of alkaline phosphatase (ALP) and osteocalcin (OCN) by upregulating their osteoblastic expression in vitro (as well as that of osterix and Runx2). Following transfection with NPY‑siRNA, the osteoblastic ability of MC3T3‑E1 cells was markedly decreased, and NPY deficiency inhibited the protein expression of osterix, Runx2, OCN and ALP in primary osteoblasts. Collectively, these results indicated that NPY played an important role in osteoblast differentiation by regulating the osterix and Runx2 pathways.
Databáze: OpenAIRE
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