Inflammatory leukocytes and iron turnover in experimental hemorrhagic lung trauma
| Autor: | Vijayalakshmi N. Ayyagari, Nikolai V. Gorbunov, James L. Atkins, Lidmila V. Asher |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Pathology medicine.medical_specialty Iron Clinical Biochemistry Hemorrhage Inflammation Pathology and Forensic Medicine Rats Sprague-Dawley Hemoglobins chemistry.chemical_compound Superoxide Dismutase-1 Antigens CD Blast Injuries Cell Movement Leukocytes medicine Animals Molecular Biology Heme Peroxidase chemistry.chemical_classification Respiratory Distress Syndrome biology Superoxide Dismutase Transferrin Degranulation Endothelial Cells Epithelial Cells Cadherins Molecular biology Extravasation Rats Pulmonary Alveoli Heme oxygenase Oxidative Stress chemistry Myeloperoxidase biology.protein Tyrosine Hemoglobin medicine.symptom Heme Oxygenase-1 |
| Zdroj: | Experimental and Molecular Pathology. 80:11-25 |
| ISSN: | 0014-4800 |
| Popis: | To monitor cascade of events following alveolar extravasation of blood due to exposure to shock wave (SW), we conducted spatiotemporal assessment of myeloperoxidase (MPO), heme oxygenase 1 (HO-1), Cu,Zn superoxide dismutase (SOD-1), transferrin (TRF), 3-nitrotyrosine (3NTyr), alveolar endothelial cadherin (VE-CDH), and the CD11b adhesion molecules on leukocytes using electron microscopy, electron paramagnetic resonance spectroscopy, immunofluorescence imaging, and immunoblotting. Accumulation of HO-1, MPO, 3NTyr, and SOD-1 in HIL at the first 12 h was associated with transmigration of inflammatory leucocytes (ILK) into hemorrhagic lesions (HLs). Biodegradation of extravasated hemoglobin (exvHb) and deposition of iron in alveoli occurred at 3-56 h post-exposure and was preceded by LKC degranulation and accumulation of MPO, HO-1, and SOD-1 in HLs. These alterations were accompanied by appearance of heme and non-heme iron complexes in HLs. A significant increase in TRF-bound [Fe(3+)] (i.e., 14.6 +/- 5.3 microM vs. 4.8 +/- 2.1 microM immediately after exposure) and non-TRF complexes of [Fe(3+)] (i.e., 4.5 +/- 1.8 microM vs. < 0.3 microM immediately after exposure) occurred at 24 h post-exposure. Transmigrations of ILK, nitroxidative stress, and iron deposition in endothelial and epithelial cells were accompanied by destruction of endothelial integrity at 3 h post-exposure, and alveolar capillary network and necrotic changes in the pulmonary epithelial cells at 24-56 h post-exposure. |
| Databáze: | OpenAIRE |
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