New electrochemiluminescent biosensors combining polyluminol and an enzymatic matrix

Autor: Loïc J. Blum, Audrey Sassolas, Béatrice D. Leca-Bouvier
Přispěvatelé: Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Centre National de la Recherche Scientifique (CNRS)-École Supérieure Chimie Physique Électronique de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2009
Předmět:
MESH: Hydrogen-Ion Concentration
Working electrode
MESH: Enzymes
Immobilized
Polymers
MESH: Chemiluminescent Measurements
Analytical chemistry
Biosensing Techniques
02 engineering and technology
Sensitivity and Specificity
01 natural sciences
Biochemistry
MESH: Alcohol Oxidoreductases
Choline
Analytical Chemistry
Luminol
chemistry.chemical_compound
Electrochemistry
Electrochemiluminescence
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Electrodes
ComputingMilieux_MISCELLANEOUS
MESH: Electrochemistry
MESH: Luminol
010401 analytical chemistry
MESH: Choline
Reproducibility of Results
Hydrogen Peroxide
Choline oxidase
MESH: Electrodes
Hydrogen-Ion Concentration
Enzymes
Immobilized
021001 nanoscience & nanotechnology
MESH: Sensitivity and Specificity
0104 chemical sciences
MESH: Polymers
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
MESH: Reproducibility of Results
Alcohol Oxidoreductases
chemistry
Polymerization
Luminescent Measurements
Luminophore
MESH: Hydrogen Peroxide
Cyclic voltammetry
0210 nano-technology
Biosensor
MESH: Biosensing Techniques
Nuclear chemistry
Zdroj: Analytical and Bioanalytical Chemistry
Analytical and Bioanalytical Chemistry, Springer Verlag, 2009, 394, pp.971
Analytical and Bioanalytical Chemistry, Springer Verlag, 2009, 394 (4), pp.971-80. ⟨10.1007/s00216-009-2780-2⟩
ISSN: 1618-2642
1618-2650
DOI: 10.1007/s00216-009-2780-2⟩
Popis: International audience; Performant reagentless electrochemiluminescent (ECL) (bio)sensors have been developed using polymeric luminol as the luminophore. The polyluminol film is obtained by cyclic voltammetry (CV) on a screen-printed electrode either in a commonly used H(2)SO(4) medium or under more original near-neutral buffered conditions. ECL responses obtained after performing polymerization either at acidic pH or at pH 6 have been compared. It appears that polyluminol formed in near-neutral medium gives the best responses for hydrogen peroxide detection. Polymerization at pH 6 by cyclic voltammetry gives a linear range extending from 8 x 10(-8) to 1.3 x 10(-4) M H(2)O(2) concentrations. Based on this performant sensor for hydrogen peroxide detection, an enzymatic biosensor has been developed by associating the polyluminol film with an H(2)O(2)-producing oxidase. Here, choline oxidase (ChOD) has been chosen as a model enzyme. To develop the biosensor, luminol has been polymerized at pH 6 by CV, and then an enzyme-entrapping matrix has been formed on the above modified working electrode. Different biological (chitosan, agarose, and alginate) and chemical (silica gels, photopolymers, or reticulated matrices) gels have been tested. Best performances have been obtained by associating a ChOD-immobilizing photopolymer with the polyluminol film. In this case, choline can be detected with a linear range extending from 8 x 10(-8) to 1.3 x 10(-4) M.
Databáze: OpenAIRE
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