The superantigen-induced polarization of T cells in rat peripheral lymph nodes is influenced by genetic polymorphisms in the IL-4 and IL-6 gene clusters
| Autor: | Lothar Rink, Marc Lörchner, Reinhard Pabst, Kurt Wonigeit, Joachim Hundrieser, Silke Overbeck, Ulrike Bode |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_treatment
T cell T-Lymphocytes Immunology Biology Immunophenotyping Antigen Species Specificity medicine Superantigen Immunology and Allergy Animals Interleukin 4 Cell Proliferation Antigens Bacterial Polymorphism Genetic Superantigens Cell growth Interleukin-6 Proteins General Medicine T lymphocyte Dendritic cell Dendritic Cells Molecular biology Rats Cytokine medicine.anatomical_structure Rats Inbred Lew Interleukin-4 Lymph Nodes |
| Zdroj: | International immunology. 19(1) |
| ISSN: | 0953-8178 |
| Popis: | In recent years, it has become clear that the polarization of T cells depends on the genetic background. However, due to the complexity of the genetic background of each animal, a direct comparison of the phenotype is difficult. In this study, a new rat strain LEW.BN-4-10 carrying the chromosomal regions on chromosomes 4 and 10, which harbor IL-6 and IL-4 gene clusters of BN, has been bred on the genetic background of LEW. It was asked whether these two gene clusters influence the polarization of T cell responses. As a model, the Mycoplasma arthritidis mitogen (MAM)-induced inflammation was used focusing on the microenvironment of the draining lymph node (LN). The effect of differences in these regions was tested by comparing LEW.BN-4-10 and LEW rats under steady-state conditions and upon injection of MAM into the forepaw. Under steady-state conditions, the two strains showed differences in the dendritic cell (DC) subset composition. When MAM was injected, the number of T cells in LEW.BN-4-10 rats producing T(h)2 cytokines such as IL-4 and IL-13 was significantly increased compared with LEW. The data suggest that these differences in the microenvironments in LN of LEW and LEW.BN-4-10 rats resulted in different susceptibility to the disease (increase of cells in LN and paw swelling). In addition, deviations in the distribution and function of injected effector T cells were found in the LN of LEW and LEW.BN-4-10 rats after MAM treatment. The data indicate that the IL-6 and IL-4 gene clusters are involved in polarizing T cell responses in vivo. |
| Databáze: | OpenAIRE |
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