Crystals of β-Xylanase from Aspergillus oryzae
| Autor: | Richard W. Pickersgill, A. M. Golubev, A. Yu. Kilimnik, K. N. Neustroev |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Endo-1
4-beta Xylanases Glycoside Hydrolases biology Aspergillus oryzae Polyethylene glycol Crystal structure biology.organism_classification Polyethylene Glycols law.invention Kinetics chemistry.chemical_compound Crystallography Isoelectric point chemistry Biochemistry Structural Biology law Xylanase Glycoside hydrolase Crystallization Molecular Biology Monoclinic crystal system |
| Zdroj: | Journal of Molecular Biology. 230:661-663 |
| ISSN: | 0022-2836 |
| Popis: | An endo-xylanase was isolated from the culture of fungus Aspergillus oryzae variant D5. The purified enzyme had a molecular weight of 24,000 and the isoelectric point of 3.6. Xylanase crystals were obtained from a polyethylene glycol 6000 solution by the hanging-drop method. Seeding was used for the enlargement of the crystal size. Crystals belong to the monoclinic space group P2(1) with cell dimensions a = 54.9 A, b = 74.5 A, c = 50.8 A, and beta = 108.7 degrees. Crystals diffract beyond 2.5 A resolution. |
| Databáze: | OpenAIRE |
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