The Cyclin-dependent Kinase Inhibitors p57 and p27 Regulate Neuronal Migration in the Developing Mouse Neocortex
| Autor: | Yasuhiro Itoh, Norihisa Masuyama, Yukiko Gotoh, Keiko Nakayama, Keiichi I. Nakayama |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Cell cycle checkpoint Cellular differentiation Green Fluorescent Proteins Neocortex Biology Biochemistry Mice Cell Movement RNA interference Cyclin-dependent kinase medicine Animals Cyclin-Dependent Kinase Inhibitor p57 Molecular Biology Neurons Messenger RNA Gene knockdown Cell Differentiation Cell Biology Anatomy Immunohistochemistry Cell biology Mice Inbred C57BL Neuroepithelial cell medicine.anatomical_structure nervous system biology.protein Female RNA Interference Cyclin-Dependent Kinase Inhibitor p27 Plasmids |
| Zdroj: | Journal of Biological Chemistry. 282:390-396 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m609944200 |
| Popis: | Neuronal precursors remain in the proliferative zone of the developing mammalian neocortex until after they have undergone neuronal differentiation and cell cycle arrest. The newborn neurons then migrate away from the proliferative zone and enter the cortical plate. The molecules that coordinate migration with neuronal differentiation have been unclear. We have proposed in this study that the cdk inhibitors p57 and p27 play a role in this coordination. We have found that p57 and p27 mRNA increase upon neuronal differentiation of neocortical neuroepithelial cells. Knockdown of p57 by RNA interference resulted in a significant delay in the migration of neurons that entered the cortical plate but did not affect neuronal differentiation. Knockdown of p27 also inhibits neuronal migration in the intermediate zone as well as in the cortical plate, as reported by others. We have also found that knockdown of p27 increases p57 mRNA levels. These results suggest that both p57 and p27 play essential roles in neuronal migration and may, in concert, coordinate the timing of neuronal differentiation, migration, and possibly cell cycle arrest in neocortical development. |
| Databáze: | OpenAIRE |
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