Prognostic value of HPV 16/18 genotyping and geminin mRNA quantification in low-grade cervical squamous intraepithelial lesion
| Autor: | Zheng, Ziwen, Yang, Xiaorong, Yao, Xinyu, Li, Ling |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Adult
Genotype Squamous Intraepithelial Lesions Uterine Cervical Neoplasms Bioengineering cervical intraepithelial neoplasia loop electrosurgical excision procedure Applied Microbiology and Biotechnology Cell Line Tumor Humans RNA Messenger Aged hpv16/18 Human papillomavirus 16 Human papillomavirus 18 Geminin General Medicine Middle Aged Prognosis Uterine Cervical Dysplasia Gene Expression Regulation Neoplastic Logistic Models ROC Curve Multivariate Analysis embryonic structures Disease Progression lsil Female TP248.13-248.65 Research Article Research Paper Follow-Up Studies Biotechnology |
| Zdroj: | Bioengineered, Vol 12, Iss 2, Pp 11482-11489 (2021) Bioengineered article-version (VoR) Version of Record |
| ISSN: | 2165-5987 2165-5979 |
| Popis: | Low-grade cervical squamous intraepithelial lesion is a precancerous neoplasia that has appreciable probability to evolve into malignancy. To explore the prognostic value of HPV 16/18 genotyping and geminin mRNA quantification in predicting the progressiveness of LSIL. We recruited 212 participants who were negative for intraepithelial lesion or malignancy (NILM 76), low-grade squamous intraepithelial lesion (LSIL 85), high-grade squamous intraepithelial lesion (HSIL 36) and cervical intraepithelial neoplasia grade cervical cancer grade 3, (CIN3 15) patients. Tissues were obtained during excisional treatment. HPV 16/18 genotyping and geminin mRNA qRT-PCR were performed. HPV 16/18 positivity rate and geminin mRNA level were integrated with the clinical parameters into a multivariate logistic model. Area under curve was yielded based on receiver operation curve derived from this multivariate logistic model. Follow-up visits were performed to LSIL patients with progression. HSIL patients have higher HPV 16/18 positivity rate and geminin mRNA levels than LSIL. Among HSIL, CIN3 have higher HPV 16/18 positivity rate and geminin mRNA levels. Multivariate logistic analysis showed that HPV 16/18 positivity and geminin mRNA expression status are independent factors for differentiating HSIL and LSIL. The baseline HPV 16/18 positivity rate and geminin mRNA levels of 18 LSIL patients who developed HSIL are significantly higher than non-progressive LSIL patients. The values examined at follow-up timepoints were also higher than baseline. These results suggest that geminin is implicated in the progression of LSIL and combining HPV 16/18 genotyping and geminin mRNA qRT-PCR could potentially differentiating the progressive LSIL and improve the efficacy of clinical intervention. |
| Databáze: | OpenAIRE |
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