Phosphatidylcholines from Pieris brassicae eggs activate an immune response in Arabidopsis
| Autor: | Philippe Reymond, Jean-Luc Wolfender, Howard Riezman, Théo Brillatz, Olivier Hilfiker, Elia Stahl, Laurence Marcourt, Isabelle Riezman, Emerson Ferreira Queiroz, André Schmiesing |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Programmed cell death QH301-705.5 media_common.quotation_subject Science insect eggs Insect Pieris brassicae 01 natural sciences General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Immune system plant defense Arabidopsis Gene expression Plant defense against herbivory Biology (General) innate immunity media_common Innate immune system General Immunology and Microbiology biology Chemistry General Neuroscience food and beverages General Medicine biology.organism_classification phosphatidylcholines Cell biology 030104 developmental biology Medicine 010606 plant biology & botany |
| Zdroj: | eLife, Vol 9 (2020) eLife, vol. 9, pp. e60293 |
| Popis: | Recognition of conserved microbial molecules activates immune responses in plants, a process termed pattern-triggered immunity (PTI). Similarly, insect eggs trigger defenses that impede egg development or attract predators, but information on the nature of egg-associated elicitors is scarce. We performed an unbiased bioactivity-guided fractionation of eggs of the butterfly Pieris brassicae. Nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry of active fractions led to the identification of phosphatidylcholines (PCs). PCs are released from insect eggs, and they induce salicylic acid and H2O2 accumulation, defense gene expression and cell death in Arabidopsis, all of which constitute a hallmark of PTI. Active PCs contain primarily C16 to C18-fatty acyl chains with various levels of desaturation, suggesting a relatively broad ligand specificity of cell-surface receptor(s). The finding of PCs as egg-associated molecular patterns (EAMPs) illustrates the acute ability of plants to detect conserved immunogenic patterns from their enemies, even from seemingly passive structures such as eggs. |
| Databáze: | OpenAIRE |
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