Interval Vibration Reduces Orthodontic Pain Via a Mechanism Involving Down-regulation of TRPV1 and CGRP
| Autor: | Thunwa Binlateh, Aunwaya Kaewpitak, Chidchanok Leethanakul, Peungchaleoy Thammanichanon |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Molar
Calcitonin Cancer Research Calcitonin Gene-Related Peptide TRPV1 Down-Regulation Pain TRPV Cation Channels Calcitonin gene-related peptide Vibration General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Transient receptor potential channel 0302 clinical medicine Downregulation and upregulation Medicine Animals Rats Wistar Receptor Pharmacology Orthodontics business.industry Rats nervous system 030220 oncology & carcinogenesis business Research Article |
| Zdroj: | In Vivo |
| Popis: | Background/aim The transient receptor potential vanilloid 1 (TRPV1) ion receptor is involved in the release of calcitonin gene-related peptide (CGRP), a major contributor to orthodontic pain. Approaches that attenuate expression of TRPV1 and CGRP may reduce orthodontic pain. We explored the ability of high-frequency interval vibration to reduce orthodontic pain. Materials and methods Orthodontic force (50 g) was applied to both maxillary first molars in 8-week-old Wistar rats (n=72). Vibration was applied at 125 Hz for 15 min/day. Duration of face grooming was assessed as a measure of orthodontic pain. Immunofluorescence and western blotting were used to assess TRPV1 and CGRP in the trigeminal ganglia. Results Compared to orthodontic force alone, application of vibration significantly decreased the duration of face grooming at 24 h and day 3 and reduced expression of TRPV1 and CGRP at 24 h. Conclusion Vibration represents a promising mechanical approach to reduce orthodontic pain. |
| Databáze: | OpenAIRE |
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