Evaluation of serum and gingival crevicular fluid microRNA-223, microRNA-203 and microRNA-200b expression in chronic periodontitis patients with and without diabetes type 2
| Autor: | Khalda Amr, Abeer I. Abd El Fattah, Ola Elazazy, Maha Rashed Abouzaid |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Type 2 diabetes Gastroenterology Pathogenesis 03 medical and health sciences 0302 clinical medicine mir-223 Internal medicine Diabetes mellitus microRNA Medicine Humans General Dentistry business.industry Tumor Necrosis Factor-alpha 030206 dentistry Cell Biology General Medicine Gingival Crevicular Fluid medicine.disease Chronic periodontitis MicroRNAs 030104 developmental biology Otorhinolaryngology Diabetes Mellitus Type 2 Chronic Periodontitis Tumor necrosis factor alpha business miR-203 |
| Zdroj: | Archives of oral biology. 121 |
| ISSN: | 1879-1506 |
| Popis: | microRNA dysregulation is a reported feature of multiple pathologies, including periodontal disease, as demonstrated on cell lines, in animal models, and tissues biopsies, but serum and gingival crevicular fluid microRNA expression data in humans is scarce, especially with the diabetes (type 2) systemic complication. Objective To assess serum and gingival crevicular fluid relative quantification levels of miR-223, miR-203, and miR-200b in chronic periodontitis and type 2 diabetic chronic periodontitis patients to address their possible implication in chronic periodontitis pathogenesis and its systemic complications and also to correlate their differential expression with some inflammatory (serum tumor necrosis factor-α and interleukin-10) parameters. Methods Sixty subjects were recruited and divided into three groups; chronic periodontitis (n = 20), type 2 diabetic chronic periodontitis (n = 20), and healthy control (n = 20). Both serum and gingival crevicular fluid were collected from each participant for miRNA expression analysis and serum inflammatory parameters assessment. Results A significant increase in the relative quantification levels of miR-223 and miR-200b were detected in patient groups along with a positive correlation with tumor necrosis factor-α. However, miR-203 was significantly decreased in patient groups associated with a negative correlation with tumor necrosis factor-α. Conclusions miR-223 and miR-200b have a potential role in chronic periodontitis pathogenesis associated with type 2 diabetes, with the ability to induce tumor necrosis factor-α secretion, while miR-203 might have a protective and healing role due to the negative correlation with the serum tumor necrosis factor-α levels found. Therefore, they may be considered as a promising therapeutic target and effective serum disease biomarkers. |
| Databáze: | OpenAIRE |
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